The response to extracytoplasmic stress in Escherichia coli is controlled by partially overlapping pathways

被引:79
作者
Connolly, L
Penas, AD
Alba, BM
Gross, CA
机构
[1] UNIV CALIF SAN FRANCISCO, DEPT BIOCHEM & BIOPHYS, SAN FRANCISCO, CA 94143 USA
[2] UNIV CALIF SAN FRANCISCO, DEPT STOMATOL, SAN FRANCISCO, CA 94143 USA
[3] UNIV CALIF SAN FRANCISCO, DEPT MICROBIOL, SAN FRANCISCO, CA 94143 USA
[4] UNIV WISCONSIN, DEPT BACTERIOL, MADISON, WI 53706 USA
关键词
rpoE; stress response; cpxRA; outer membrane; nlpE; periplasm;
D O I
10.1101/gad.11.15.2012
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The activity of the alternate sigma-factor sigma(E) of Escherichia coli is induced by several stressors that lead to the extracytoplasmic accumulation of misfolded or unfolded protein. The sigma(E) regulon contains several genes, including that encoding the periplasmic protease DegP, whose products are thought to be required for maintaining the integrity of the cell envelope because cells lacking sigma(E) are sensitive to elevated temperature and hydrophobic agents. Selection of multicopy suppressors of the temperature-sensitive phenotype of cells lacking sigma(E) revealed that overexpression of the lipoprotein NlpE restored high temperature growth to these cells. Overexpression of NlpE has been shown previously to induce DegP synthesis by activating the Cpx two-component signal transduction pathway, and suppression of the temperature-sensitive phenotype by NlpE was found to be dependent on the Cpx proteins. In addition, a constitutively active form of the CpxA sensor/kinase also fully suppressed the temperature-sensitive defect of cells lacking sigma(E). DegP was found to be necessary, but not sufficient, for suppression. Activation of the Cpx pathway has also been shown to alleviate the toxicity of several LamB mutant proteins. Together, these results reveal the existence of two partially overlapping regulatory systems involved in the response to extracytoplasmic stress in E. coli.
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页码:2012 / 2021
页数:10
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