Temporal Control of Retroviral Transgene Expression in Newborn Cells in the Adult Brain

被引：9

作者：

Braun, Simon M. G. ^{[1
,2
,3
,4
]}

Machado, Raquel A. C. ^{[1
,2
]}

Jessberger, Sebastian ^{[1
,2
,3
,4
]}

机构：

[1] Univ Zurich, Brain Res Inst, Fac Med, CH-8057 Zurich, Switzerland

[2] Swiss Fed Inst Technol, Inst Mol Hlth Sci, Dept Biol, CH-8093 Zurich, Switzerland

[3] Univ Zurich, Neurosci Ctr Zurich, CH-8057 Zurich, Switzerland

[4] Swiss Fed Inst Technol, CH-8057 Zurich, Switzerland

来源：

STEM CELL REPORTS | 2013年 / 1卷 / 02期

基金：

瑞士国家科学基金会;

关键词：

NEURAL STEM-CELLS; HIPPOCAMPAL NEUROGENESIS; NEURONS; MATURATION; IDENTITY; PROX1;

D O I：

10.1016/j.stemcr.2013.06.003

中图分类号：

Q813 [细胞工程];

学科分类号：

100113 [医学细胞生物学];

摘要：

Neural stem/progenitor cells (NSPCs) generate new neurons throughout life in distinct areas of the adult mammalian brain. Besides classical transgenesis-based approaches, retrovirus-mediated genetic manipulation is frequently used to study mechanisms that regulate neurogenesis in the nervous system. Here, we show that fusion of a tamoxifen-regulatable estrogen receptor (ERT2) motif to transcription factors (i.e., ASCL1 and NEUROD1) enables temporal control of transgene expression in adult mouse NSPCs in vitro and in vivo. Thus, the approach described here represents a versatile strategy for regulating gene expression to study gene function in dividing cells and their progeny.

引用

收藏

页码：114 / 122

页数：9

相关论文

共 23 条

[1]

Enriched Monolayer Precursor Cell Cultures from Micro-Dissected Adult Mouse Dentate Gyrus Yield Functional Granule Cell-Like Neurons [J].

Babu, Harish ;

Cheung, Giselle ;

Kettenmann, Helmut ;

Palmer, Theo D. ;

Kempermann, Gerd .

PLOS ONE, 2007, 2 (04)

[2]

Gene Expression Profiling of Neural Stem Cells and Their Neuronal Progeny Reveals IGF2 as a Regulator of Adult Hippocampal Neurogenesis [J].

Bracko, Oliver ;

Singer, Tatjana ;

Aigner, Stefan ;

Knobloch, Marlen ;

Winner, Beate ;

Ray, Jasodhara ;

Clemenson, Gregory D., Jr. ;

Suh, Hoonkyo ;

Couillard-Despres, Sebastien ;

Aigner, Ludwig ;

Gage, Fred H. ;

Jessberger, Sebastian .

JOURNAL OF NEUROSCIENCE, 2012, 32 (10) :3376-3387

[3]

Proneural bHLH and Brn proteins coregulate a neurogenic program through cooperative binding to a conserved DNA motif [J].

Castro, Diogo S. ;

Skowronska-Krawczyk, Dorota ;

Armant, Olivier ;

Donaldson, Ian J. ;

Parras, Carlos ;

Hunt, Charles ;

Critchley, James A. ;

Nguyen, Laurent ;

Gossler, Achim ;

Goettgens, Berthold ;

Matter, Jean-Marc ;

Guillemot, Francois .

DEVELOPMENTAL CELL, 2006, 11 (06) :831-844

[4]

Visualization and genetic manipulation of adult neurogenesis using transgenic mice [J].

Dhaliwal, Jagroop ;

Lagace, Diane C. .

EUROPEAN JOURNAL OF NEUROSCIENCE, 2011, 33 (06) :1025-1036

[5]

Neurod1 is essential for the survival and maturation of adult-born neurons [J].

Gao, Zhengliang ;

Ure, Kerstin ;

Ables, Jessica L. ;

Lagace, Diane C. ;

Nave, Klaus-Armin ;

Goebbels, Sandra ;

Eisch, Amelia J. ;

Hsieh, Jenny .

NATURE NEUROSCIENCE, 2009, 12 (09) :1090-1092

[6]

GABA regulates synaptic integration of newly generated neurons in the adult brain [J].

Ge, SY ;

Goh, ELK ;

Sailor, KA ;

Kitabatake, Y ;

Ming, GL ;

Song, HJ .

NATURE, 2006, 439 (7076) :589-593

[7]

Directing Astroglia from the Cerebral Cortex into Subtype Specific Functional Neurons [J].

Heinrich, Christophe ;

Blum, Robert ;

Gascon, Sergio ;

Masserdotti, Giacomo ;

Tripathi, Pratibha ;

Sanchez, Rodrigo ;

Tiedt, Steffen ;

Schroeder, Timm ;

Goetz, Magdalena ;

Berninger, Benedikt .

PLOS BIOLOGY, 2010, 8 (05)

[8]

Persistent Sonic Hedgehog Signaling in Adult Brain Determines Neural Stem Cell Positional Identity [J].

Ihrie, Rebecca A. ;

Shah, Jugal K. ;

Harwell, Corey C. ;

Levine, Jacob H. ;

Guinto, Cristina D. ;

Lezameta, Melissa ;

Kriegstein, Arnold R. ;

Alvarez-Buylla, Arturo .

NEURON, 2011, 71 (02) :250-262

[9]

Temporally-controlled site-specific mutagenesis in the basal layer of the epidermis:: comparison of the recombinase activity of the tamoxifen-inducible Cre-ERT and Cre-ERT2 recombinases [J].

Indra, AK ;

Warot, X ;

Brocard, J ;

Bornert, JM ;

Xiao, JH ;

Chambon, P ;

Metzger, D .

NUCLEIC ACIDS RESEARCH, 1999, 27 (22) :4324-4327

[10]

Prox1 postmitotically defines dentate gyrus cells by specifying granule cell identity over CA3 pyramidal cell fate in the hippocampus [J].

Iwano, Tomohiko ;

Masuda, Aki ;

Kiyonari, Hiroshi ;

Enomoto, Hideki ;

Matsuzaki, Fumio .

DEVELOPMENT, 2012, 139 (16) :3051-3062