Role of urinary trypsin inhibitor in the maintenance of pregnancy in mice

被引:32
作者
Kaga, N
Katsuki, Y
Futamura, Y
Obata, M
Shibutani, Y
机构
[1] Toxicology Laboratory, Mochida Pharmaceutical Co., Ltd., Shizuoka
[2] Toxicology Laboratory, Mochida Pharmaceutical Company, Ltd., Shizuoka 426, 342 Gensuke, Fujieda
关键词
D O I
10.1016/0029-7844(96)00268-2
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Objective: To investigate the mechanisms whereby urinary trypsin inhibitor prevents lipopolysaccharide-induced preterm delivery in mice. Methods: On day 15 of pregnancy, C3H/HeNCrg female mice impregnated by Crg:B6D2F1 male mice were treated intraperitoneally with lipopolysaccharide (50 mu/kg, twice at a 3-hour interval) to induce preterm deliver. Urinary trypsin inhibitor (2.5 x 10(4), 7.5 x 10(4), or 25 x 10(4) units/kg, ten times at 1-hour intervals) or saline solution was administered intraperitoneally to the animals. Results: The incidence of preterm delivery was significantly decreased on a dose-related basis by urinary trypsin inhibitor treatment. Urinary trypsin inhibitor prevented the morphologic and functional changes in fetal membranes and cervical ripening preceding the onset of preterm delivery. Urinary trypsin inhibitor also suppressed the increase in plasma and amniotic fluid concentrations of interleukin-1 alpha, interleukin-6, and tumor necrosis factor-alpha after the lipopolysaccharide dosing in this animal model for preterm delivery. Conclusion: Urinary trypsin inhibitor prevents the pathogenicity of preterm delivery through the suppression of cytokine production.
引用
收藏
页码:872 / 882
页数:11
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