Studying the interactome with the yeast two-hybrid system and mass spectrometry

被引：47

作者：

Causler, B ^{[1
]}

机构：

[1] Univ Leeds, Sch Biol, Leeds LS2 9JT, W Yorkshire, England

来源：

MASS SPECTROMETRY REVIEWS | 2004年 / 23卷 / 05期

关键词：

interactome; mass spectrometry; protein-protein interaction; proteomics; yeast two-hybrid;

D O I：

10.1002/mas.10080

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

Protein interactions are crucial to the life of a cell. The analysis of such interactions is allowing biologists to determine the function of uncharacterized proteins and the genes that encode them. The yeast two-hybrid system has become one of the most popular and powerful tools to study protein-protein interactions. With the advent of proteomics, the two-hybrid system has found a niche in interactome mapping. However it is clear that only by combining two-hybrid data with that from complementary approaches such as mass spectrometry (MS) can the interactome be analyzed in full. This review introduces the yeast two-hybrid system to those unfamiliar with the technique, and discusses how it can be used in combination with MS to unravel the network of protein interactions that occur in a cell. (C) 2004 Wiley Periodicals, Inc.

引用

页码：350 / 367

页数：18

共 81 条

[1] Mass spectrometry-based proteomics [J].

Aebersold, R ;

Mann, M .

NATURE, 2003, 422 (6928) :198-207

[2] Isolation of an AP-1 repressor by a novel method for detecting protein-protein interactions [J].

Aronheim, A ;

Zandi, E ;

Hennemann, H ;

Elledge, SJ ;

Karin, M .

MOLECULAR AND CELLULAR BIOLOGY, 1997, 17 (06) :3094-3102

[3]

Auerbach D, 2002, PROTEOMICS, V2, P611, DOI 10.1002/1615-9861(200206)2:6<611::AID-PROT611>3.0.CO

[4]

2-Y

[5]

Bader GD, 2003, NUCLEIC ACIDS RES, V31, P248, DOI 10.1093/nar/gkg056

[6] Analyzing yeast protein-protein interaction data obtained from different sources [J].

Bader, GD ;

Hogue, CWV .

NATURE BIOTECHNOLOGY, 2002, 20 (10) :991-997

[7] A protein linkage map of Escherichia coli bacteriophage T7 [J].

Bartel, PL ;

Roecklein, JA ;

SenGupta, D ;

Fields, S .

NATURE GENETICS, 1996, 12 (01) :72-77

[8] Affinity purification-mass spectrometry - Powerful tools for the characterization of protein complexes [J].

Bauer, A ;

Kuster, B .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 2003, 270 (04) :570-578

[9] A proteomics strategy to elucidate functional protein-protein interactions applied to EGF signaling [J].

Blagoev, B ;

Kratchmarova, I ;

Ong, SE ;

Nielsen, M ;

Foster, LJ ;

Mann, M .

NATURE BIOTECHNOLOGY, 2003, 21 (03) :315-318

[10] A BACTERIAL REPRESSOR PROTEIN OR A YEAST TRANSCRIPTIONAL TERMINATOR CAN BLOCK UPSTREAM ACTIVATION OF A YEAST GENE [J].

BRENT, R ;

PTASHNE, M .

NATURE, 1984, 312 (5995) :612-615

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