Studying the interactome with the yeast two-hybrid system and mass spectrometry

被引：47

作者：

Causler, B ^{[1
]}

机构：

[1] Univ Leeds, Sch Biol, Leeds LS2 9JT, W Yorkshire, England

来源：

MASS SPECTROMETRY REVIEWS | 2004年 / 23卷 / 05期

关键词：

interactome; mass spectrometry; protein-protein interaction; proteomics; yeast two-hybrid;

D O I：

10.1002/mas.10080

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

Protein interactions are crucial to the life of a cell. The analysis of such interactions is allowing biologists to determine the function of uncharacterized proteins and the genes that encode them. The yeast two-hybrid system has become one of the most popular and powerful tools to study protein-protein interactions. With the advent of proteomics, the two-hybrid system has found a niche in interactome mapping. However it is clear that only by combining two-hybrid data with that from complementary approaches such as mass spectrometry (MS) can the interactome be analyzed in full. This review introduces the yeast two-hybrid system to those unfamiliar with the technique, and discusses how it can be used in combination with MS to unravel the network of protein interactions that occur in a cell. (C) 2004 Wiley Periodicals, Inc.

引用

页码：350 / 367

页数：18

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