Ascorbic acid reaction with disulphide compounds: effects and applications

被引：5

作者：

Baker, WL ^{[1
]}

机构：

[1] Swinburne Univ Technol, Sch Sci & Engn, Hawthorn, Vic 3122, Australia

来源：

TALANTA | 2000年 / 52卷 / 03期

关键词：

disulphides; cystine; glutathione disulphide; hydrogen peroxide; copper complexes;

D O I：

10.1016/S0039-9140(00)00360-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method was developed for estimation of concentrations of cystine based on the reaction of this oxidised amino acid with ascorbic acid and copper ion. The standard curve which was constructed was used as the basis for investigation of the effects which both cystine and glutathione disulphide, and some disulphide proteins, exerted on the amount of hydrogen peroxide formed in the reaction of ascorbic acid. The hydrogen peroxide product was lowered in the presence of the disulphide compounds. The disulphide compounds also lowered the amount of deoxyribose reacting material formed during the ascorbic acid oxidation. The reaction between ascorbic acid and copper and cystine was used to estimate the amount of the disulphide amino acid in acid digests of proteins. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：425 / 433

页数：9

共 23 条

[11] DETERMINATION OF ASCORBIC-ACID IN PHARMACEUTICALS USING DIRECT ULTRAVIOLET SPECTROPHOTOMETRY [J].

LAU, OW ;

LUK, SF ;

WONG, KS .

ANALYST, 1987, 112 (07) :1023-1025

[12]

LIU WK, 1971, J MEIENHOFER BIOCH J, V10, P2849

[13] GLUTATHIONE [J].

MEISTER, A ;

ANDERSON, ME .

ANNUAL REVIEW OF BIOCHEMISTRY, 1983, 52 :711-760

[14]

PRUTZ WA, 1994, BIOCHEM J, V302, P373

[15] FORMATION OF HYDROXYL RADICALS FROM HYDROGEN-PEROXIDE IN THE PRESENCE OF IRON - IS HEMOGLOBIN A BIOLOGICAL FENTON REAGENT [J].

PUPPO, A ;

HALLIWELL, B .

BIOCHEMICAL JOURNAL, 1988, 249 (01) :185-190

[16] INACTIVATION OF DOPAMINE BETA-MONOOXYGENASE BY HYDROGEN-PEROXIDE AND BY ASCORBATE [J].

SKOTLAND, T ;

LJONES, T .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1980, 201 (01) :81-87

[17] DIRECT SPECTROPHOTOMETRIC DETECTION OF ASCORBATE FREE-RADICAL FORMED BY DOPAMINE BETA-MONOOXYGENASE AND BY ASCORBATE OXIDASE [J].

SKOTLAND, T ;

LJONES, T .

BIOCHIMICA ET BIOPHYSICA ACTA, 1980, 630 (01) :30-35

[18] MICROIODOMETRIC DETERMINATION OF BETA-LACTAMASE ACTIVITY [J].

SYKES, RB ;

NORDSTRO.K .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1972, 1 (02) :94-&

[19]

TAQUIKHAN MM, 1967, J AM CHEM SOC, V89, P4176

[20] FLUORESCAMINE - REAGENT FOR ASSAY OF AMINO-ACIDS, PEPTIDES, PROTEINS, AND PRIMARY AMINES IN PICOMOLE RANGE [J].

UDENFRIEND, S ;

STEIN, S ;

BOHLEN, P ;

DAIRMAN, W .

SCIENCE, 1972, 178 (4063) :871-+

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