Comparison of liposome amplification and fluorophor detection in flow-injection immunoanalyses

被引：21

作者：

Lee, MY ^{[1
]}

Durst, RA ^{[1
]}

Wong, RB ^{[1
]}

机构：

[1] AMER CYANAMID CO,AGR RES CTR,PRINCETON,NJ 08543

来源：

ANALYTICA CHIMICA ACTA | 1997年 / 354卷 / 1-3期

关键词：

liposome; imazethapyr; immunoanalysis; flow-injection liposome immunoanalysis (FILIA);

D O I：

10.1016/S0003-2670(97)00448-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A flow-injection liposome immunoanalysis (FILIA) system was developed for the measurement of imazethapyr herbicide. The liposome is a spherical vesicle encapsulating many marker molecules in an aqueous interior. By incorporating analyte-phospholipid conjugates into the bilayer, the liposome can competitively bind to anti-analyte antibody, immobilized in the immunoreactor column of the FILIA system. In this study, the analyte-tagged liposome containing a fluorescent dye, ng carboxyfluorescein, was compared to a single fluorophor-tagged analyte used for the generation of the analytical signal. The liposome enhanced sensitivity by 1000-fold compared to the single fluorescent molecule-tagged analyte. This is the first report to demonstrate directly the amplification of the response by liposomes in a flow-injection immunoassay. (C) 1997 Elsevier Science B.V.

引用

页码：23 / 28

页数：6