Binding behaviour of pyrimethanil-imprinted polymers prepared in the presence of polar co-monomers

被引:7
作者
Baggiani, Claudio [1 ]
Baravalle, Patrizia [1 ]
Giovannoli, Cristina [1 ]
Tozzi, Cinzia [1 ]
机构
[1] Univ Turin, Dept Analyt Chem, Lab Bioanalyt Chem, I-10125 Turin, Italy
关键词
molecular imprinting; co-monomer; molecular recognition N-(4,6-dimethylpyrimidin-2-yl)phenylamine; pyrimethanil; 4-phenylazoresorcinol; Sudan Orange G;
D O I
10.1016/j.chroma.2006.03.067
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Here, we report the evaluation of the molecular recognition properties of a small library of pyrimethanil-imprinted pellicular beads prepared in the presence of several co-monomers of decreasing hydrophobicity, namely N-methacrylamidopiperidine, methylmethacrylate, N-methacrylamidopyrrolidine, N-vinylpyrrolidone, 2-hydroxyethylmethacrylate, acrylonitrile, N-methacrylamidomorpholine, and N,N-dimethylacrylamide, The effect of the presence of these co-monomers on the molecular recognition properties of the beads were studied by eluting the pyrimethanil/Sudan Orange G pair, in the presence of acetonitrile/water mobile phases of increasing polarity. The pyrimethanil was used to probe the specific interactions due to the presence of binding sites. The Sudan Orange G (that is greatly different from the template but with comparable hydrophobicity and molecular mass) was instead used to probe the non-specific interactions due to the bulk of the polymer. The experimental results show that the polymers prepared in the presence of polar co-monomers are characterised by a marked increase of the analytes retention if compared with the imprinted polymer prepared without these co-monomers. The increase of retention is more pronounced for pyrimethanil compared to Sudan Orange G with polymers prepared in the presence of the more hydrophilic co-monomers N-vinylpyrrolidone, acrylonitrile and N-methacrylamidomorpholine, while N,N-dimethylacrylamide is the only co-monomer that does not significantly influence the retention of analytes. Increasing the amount of water in the mobile phase progressively diminishes this effect. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:74 / 80
页数:7
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