Discrimination of micromass-induced chondrocytes from human mesenchymal stem cells by focal plane array-Fourier transform infrared microspectroscopy

被引：8

作者：

Chonanant, Chirapond ^{[1
]}

Bambery, Keith R. ^{[2
]}

Jearanaikoon, Nichada ^{[3
]}

Chio-Srichan, Sirinart ^{[3
]}

Limpaiboon, Temduang ^{[4
]}

Tobin, Mark J. ^{[2
]}

Heraud, Philip ^{[5
,6
]}

Jearanaikoon, Patcharee ^{[4
]}

机构：

[1] Burapha Univ, Fac Allied Hlth Sci, Dept Med Technol, Chon Buri 20131, Thailand

[2] Australian Synchrotron, Chon Buri 20131, Thailand

[3] Synchrotron Light Res Inst, Publ Org, Nakhon Ratchasima 30000, Thailand

[4] Khon Kaen Univ, Fac Associated Med Sci, Ctr Res & Dev, Med Diagnost Labs, Khon Kaen 40002, Thailand

[5] Monash Univ, Sch Chem, Ctr Biospectroscopy, Clayton, Vic 3800, Australia

[6] Monash Univ, Dept Anat & Dev Biol, Clayton, Vic 3800, Australia

来源：

TALANTA | 2014年 / 130卷

关键词：

Human mesenchymal stem cells; Micromass culture technique; Chondrogenic differentiation; FPA-FTIR microspectroscopy; CHONDROGENIC DIFFERENTIATION; RAMAN-SPECTRA; TGF-BETA; TISSUE; CARTILAGE; SPECTROSCOPY; CULTURES; LINE; CLASSIFICATION; PROTEOGLYCAN;

D O I：

10.1016/j.talanta.2014.05.037

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学];

摘要：

Rapid and sensitive methods for identifying stem cell differentiation state are required for facilitating future stem cell therapies. We aimed to evaluate the capability of focal plane array-Fourier transform infrared (FPA-FTIR) microspectroscopy for characterising the differentiation of chondrocytes from human mesenchymal stem cells (hMSCs). Successful induction was validated by reverse transcription polymerase chain reaction (RT-PCR) and Western blot analysis for collagen and aggrecan expression as chondrocyte markers in parallel with the spectroscopy. Spectra derived from chondrocyte-induced cells revealed strong IR absorbance bands attributed to collagen near 1338 and 1234 cm(-1) and proteoglycan at 1245 and 1175-960 cm(-1) compared to the non-induced cells. In addition, spectra from control and induced cells are segregated into separate clusters in partial least squares discriminant analysis score plots at the very early stages of induction and discrimination of an independent set of validation spectra with 100% accuracy. The predominant bands responsible for this discrimination were associated with collagen and aggrecan protein concordant with those obtained from RT-PCR and Western blot techniques. Our findings support the capability of FPA-FTIR microspectroscopy as a label-free tool for stem cell characterization allowing rapid and sensitive detection of macromolecular changes during chondrogenic differentiation. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：39 / 48

页数：10

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