Direct spectroelectrochemistry of peroxidases immobilised on mesoporous metal oxide electrodes: Towards reagentless hydrogen peroxide sensing

被引：22

作者：

Astuti, Yeni ^{[1
]}

Topoglidis, Emmanuel ^{[1
]}

Cass, Anthony G. ^{[2
]}

Durrant, James R. ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Dept Chem, London SW7 2AY, England

[2] Univ London Imperial Coll Sci Technol & Med, Inst Biomed Engn, London SW7 2AY, England

来源：

ANALYTICA CHIMICA ACTA | 2009年 / 648卷 / 01期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Horseradish peroxidase; Cytochrome c peroxidase; Hydrogen peroxide; Biosensor; Titania; SnO2; electrodes; CYTOCHROME-C PEROXIDASE; HORSERADISH-PEROXIDASE; BIOSENSOR; ADSORPTION; PROTEIN; ELECTROCHEMISTRY; FORMS; FILMS;

D O I：

10.1016/j.aca.2009.06.031

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this paper, we employ two peroxidases (horseradish peroxidase, HRP and cytochrome c peroxidase, CcP) to demonstrate their ability to retain their redox and biological functions after their immobilisation on mesoporous TiO2 and SnO2 electrodes. We will also demonstrate the use of HRP immobilised on the metal oxide electrodes for the development of reagentless optical and electrochemical biosensors for the detection of hydrogen peroxide (H2O2) with low detection limit of 0.04 and 1 mu M, respectively. Crown Copyright (C) 2009 Published by Elsevier B.V. All rights reserved.

引用

页码：2 / 6

页数：5

共 35 条

[1] FAST INTERFACIAL ELECTRON-TRANSFER BETWEEN CYTOCHROME-C PEROXIDASE AND GRAPHITE-ELECTRODES PROMOTED BY AMINOGLYCOSIDES - NOVEL ELECTROENZYMIC CATALYSIS OF H2O2 REDUCTION [J].

ARMSTRONG, FA ;

LANNON, AM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1987, 109 (23) :7211-7212

[2] Proton-coupled electron transfer of flavodoxin immobilized on nanostructured tin dioxide electrodes: Thermodynamics versus kinetics control of protein redox function [J].

Astuti, Y ;

Topoglidis, E ;

Briscoe, PB ;

Fantuzzi, A ;

Gilardi, G ;

Durrant, JR .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (25) :8001-8009

[3]

Barbe CJ, 1997, J AM CERAM SOC, V80, P3157, DOI 10.1111/j.1151-2916.1997.tb03245.x

[4]

Bosshard H.R., 1991, PEROXIDASES CHEM BIO, V2, P51

[5] A one-step method to construct a third-generation biosensor based on horseradish peroxidase and gold nanoparticles embedded in silica sol-gel network on gold modified electrode [J].

Di, JW ;

Shen, CP ;

Peng, SH ;

Tu, YF ;

Li, SJ .

ANALYTICA CHIMICA ACTA, 2005, 553 (1-2) :196-200

[6]

Dunford H.B., 1991, PEROXIDASES CHEM BIO, VII, P1

[7] Adsorption of differently charged forms of horseradish peroxidase on metal electrodes of different nature:: effect of surface charges [J].

Ferapontova, E ;

Domínguez, E .

BIOELECTROCHEMISTRY, 2002, 55 (1-2) :127-130

[8] Effect of pH on direct electron transfer in the system gold electrode-recombinant horseradish peroxidase [J].

Ferapontova, E ;

Gorton, L .

BIOELECTROCHEMISTRY, 2002, 55 (1-2) :83-87

[9] Effect of cysteine mutations on direct electron transfer of horseradish peroxidase on gold [J].

Ferapontova, E ;

Schmengler, K ;

Börchers, T ;

Ruzgas, T ;

Gorton, L .

BIOSENSORS & BIOELECTRONICS, 2002, 17 (11-12) :953-963

[10] Direct peroxidase bioelectrocatalysis on a variety of electrode materials [J].

Ferapontova, EE .

ELECTROANALYSIS, 2004, 16 (13-14) :1101-1112

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