Single β-Actin mRNA Detection in Neurons Reveals a Mechanism for Regulating Its Translatability

被引：216

作者：

Buxbaum, Adina R. ^{[1
,2
]}

Wu, Bin ^{[1
,2
]}

Singer, Robert H. ^{[1
,2
,3
,4
]}

机构：

[1] Albert Einstein Coll Med, Dept Anat & Struct Biol, Bronx, NY 10461 USA

[2] Albert Einstein Coll Med, Gruss Lipper Biophoton Ctr, Bronx, NY 10461 USA

[3] Albert Einstein Coll Med, Dominick P Purpura Dept Neurosci, Bronx, NY 10461 USA

[4] Howard Hughes Med Inst, Ashburn, VA 20147 USA

来源：

SCIENCE | 2014年 / 343卷 / 6169期

关键词：

LONG-TERM POTENTIATION; SYNAPTIC PLASTICITY; PROTEIN-SYNTHESIS; GRANULES; TRANSLOCATION; LOCALIZATION; RECEPTORS;

D O I：

10.1126/science.1242939

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The physical manifestation of learning and memory formation in the brain can be expressed by strengthening or weakening of synaptic connections through morphological changes. Local actin remodeling underlies some forms of plasticity and may be facilitated by local beta-actin synthesis, but dynamic information is lacking. In this work, we use single-molecule in situ hybridization to demonstrate that dendritic beta-actin messenger RNA (mRNA) and ribosomes are in a masked, neuron-specific form. Chemically induced long-term potentiation prompts transient mRNA unmasking, which depends on factors active during synaptic activity. Ribosomes and single beta-actin mRNA motility increase after stimulation, indicative of release from complexes. Hence, the single-molecule assays we developed allow for the quantification of activity-induced unmasking and availability for active translation. Further, our work demonstrates that beta-actin mRNA and ribosomes are in a masked state that is alleviated by stimulation.

引用

页码：419 / 422

页数：4

共 20 条

[1] The fragile X mental retardation protein and group I metabotropic glutamate receptors regulate levels of mRNA granules in brain [J].