Direct expression of PCR products in a cell-free transcription/translation system: synthesis of antibacterial peptide cecropin

被引：23

作者：

Martemyanov, KA ^{[1
]}

Spirin, AS ^{[1
]}

Gudkov, AT ^{[1
]}

机构：

[1] RUSSIAN ACAD SCI,INST PROT RES,PUSHCHINO 142292,MOSCOW REGION,RUSSIA

来源：

FEBS LETTERS | 1997年 / 414卷 / 02期

关键词：

transcription; translation; antibacterial peptide cecropin; polymerase chain reaction technique;

D O I：

10.1016/S0014-5793(97)01011-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and effective methodology is proposed for direct expression of PCR-generated linear DNAs in cell-free transcription/translation systems without cloning DNA fragments in plasmids. This methodology is realized for the synthesis of the active antibacterial peptide cecropin using the synthetic coding sequence. Possible scientific applications and perspectives of the proposed approach are discussed. (C) 1997 Federation of European Biochemical Societies.

引用

页码：268 / 270

页数：3

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