Sequence information from 42-108-mer DNAs (complete for a 50-mer) by tandem mass spectrometry

被引:128
作者
Little, DP [1 ]
Aaserud, DJ [1 ]
Valaskovic, GA [1 ]
McLafferty, FW [1 ]
机构
[1] CORNELL UNIV,BAKER LAB,DEPT CHEM,ITHACA,NY 14853
关键词
D O I
10.1021/ja9533611
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Complete sequence information for an ''unknown'' 50-mer DNA and extensive sequence verification for another 50-mer and 42-, 51-, 55-, 60-, 72-, 100-, and 108-mer DNAs is obtained by electrospray ionization/Fourier transform mass spectrometry that supplies 10-100x higher accuracy and resolving-power data using nozzle-skimmer (NS), collisionally activated, and infrared multiphoton dissociation (IRMPD). In addition to the previously recognized 3'- and 5'-terminal (w and a) ions, internal ions (i) and MS/MS/MS of fragment ions provide unique structural information across the DNA. NS dissociation can also yield other new backbone cleavages (forming b, c, d, and r ions) that provide extensive 5'-end information. These spectra indicate that loss of the base T rarely triggers formation of w, a, or i fragment ions, a correlation of further sequencing utility. Point mutation screening is demonstrated using a modified 50-mer unknown; a 9.04 (theory 9.01) decrease in the molecular weight (M(r)) value indicates A --> while three IRMPD fragment ions pinpoint this mutation at base 27. Introduction (measurement time <1 min) of 8 x 10(-16) mol of the 50-mer gave an M(r) value with only a 0.2 error.
引用
收藏
页码:9352 / 9359
页数:8
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