Influence of deleterious rhizobacteria on leafy spurge (Euphorbia esula) roots

Rhizobacteria have been shown to be phytotoxic to leafy spurge in laboratory assays. This field study investigated the influence of two strains of Pseudomonas fluorescens [Trevisan, (Migula)], deleterious rhizobacteria (DRB), on root weight, root bud number, and root carbohydrate content of leafy spurge at three sites located in northeast and north-central South Dakota. Soils were inoculated with 2 g of starch-based granules containing no bacteria or starch granules containing 10(8) colony-forming units (cfu)/g of either bacterial strain LS102 (Montana origin) or LS174 (South Dakota origin). Bacterial strains were detected on root samples from treated areas. Root weight and root carbohydrate content were reduced about 20% compared to roots from control plots.