The biofunction of orange-spotted grouper (Epinephelus coioides) CC chemokine ligand 4 (CCL4) in innate and adaptive immunity

被引:47
作者
Hsu, Yi-Jiou [1 ]
Hou, Chia-Yi [1 ,2 ]
Lin, Shih-Jie [1 ]
Kuo, Wan-Ching [1 ]
Lin, Han-Tso [5 ]
Lin, John Han-You [1 ,3 ,4 ]
机构
[1] Natl Cheng Kung Univ, Coll Biosci & Biotechnol, Inst Biotechnol, Tainan 701, Taiwan
[2] Chi Mei Med Ctr, Tainan 736, Taiwan
[3] Natl Cheng Kung Univ, Res Ctr Agr Biotechnol, Tainan 701, Taiwan
[4] Natl Cheng Kung Univ, Ctr Biosci, Tainan 701, Taiwan
[5] Ming Chuan Univ, Dept Biotechnol, Guishan Township 333, Taoyuan County, Taiwan
关键词
Chemokines; CCL4; Epinephelus coioides; Bioactivity; CROAKER PSEUDOSCIAENA-CROCEA; TROUT ONCORHYNCHUS-MYKISS; NECROSIS VIRUS-VACCINE; INFLAMMATORY RESPONSE; VIBRIO-ANGUILLARUM; DENDRITIC CELLS; CXC CHEMOKINE; IFN-GAMMA; T-CELLS; EXPRESSION;
D O I
10.1016/j.fsi.2013.09.020
中图分类号
S9 [水产、渔业];
学科分类号
090805 [渔业资源学];
摘要
CC chemokine (motif) ligand 4 (CCL4) is indispensable to the chemoattraction of macrophages, natural killer cells, and lymphocytes in mammals; however, it has only been cloned in a limited number of fish species and information related to its biofunction remains ambiguous with regard to teleosts. To explore the role of teleost CCL4, we first evaluated the mRNA expression of the Epinepheltts coioides CCL4 (gCCL4) gene in various organs under LPS and poly (I:C) stimulated; secondary, we evaluated the immune-related genes expression of fish under the recombinant gCCL4 protein stimulated. Our results revealed an increase in the mRNA of gCCL4 in immune organs immediately following stimulation by poly (I:C); however, in LPS stimulated fish, the expression did not increase until nearly 24 h after induction. In biofunction assays, recombinant gCCL4 was found to induce chemotactic activity in the peripheral blood leukocytes of groupers and up-regulate the gene expressions of grouper TNFA1 (TNF-alpha 1), TNFA2 (TNF-alpha 2), IFNG (IFN-gamma), MX, TBX21 (1-bet), CD8 (alpha and beta chain). These findings indicate that grouper CCL4 attracts leukocytes, induces an inflammatory response, and drives lymphocyte differentiation into the Th1 pathway. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1891 / 1898
页数:8
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