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High-throughput fluorescent tagging of full-length arabidopsis gene products in planta
被引:189
作者:

Tian, GW
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机构: Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA

Mohanty, A
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机构: Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA

Chary, SN
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机构: Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA

Li, SJ
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机构: Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA

Paap, B
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h-index: 0
机构: Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA

Drakakaki, G
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h-index: 0
机构: Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA

Kopec, CD
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h-index: 0
机构: Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA

Li, JX
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机构: Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA

Ehrhardt, D
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机构: Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA

Jackson, D
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h-index: 0
机构: Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA

Rhee, SY
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h-index: 0
机构: Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA

Raikhel, NV
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机构: Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA

Citovsky, V
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机构: Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA
机构:
[1] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA
[2] Watson Sch Biol Sci, Cold Spring Harbor, NY 11724 USA
[3] Univ Calif Riverside, Ctr Plant Cell Biol, Riverside, CA 92521 USA
[4] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA
[5] Arabidopsis Informat Resource, Stanford, CA 94305 USA
[6] Carnegie Inst Sci, Stanford, CA 94305 USA
[7] SUNY Stony Brook, Dept Biochem & Cell Biol, Stony Brook, NY 11794 USA
关键词:
D O I:
10.1104/pp.104.040139
中图分类号:
Q94 [植物学];
学科分类号:
071001 ;
摘要:
We developed a high-throughput methodology, termed fluorescent tagging of full-length proteins (FTFLP), to analyze expression patterns and subcellular localization of Arabidopsis gene products in planta. Determination of these parameters is a logical first step in functional characterization of the approximately one-third of all known Arabidopsis genes that encode novel proteins of unknown function. Our FTFLP-based approach offers two significant advantages: first, it produces internally-tagged full-length proteins that are likely to exhibit native intracellular localization, and second, it yields information about the tissue specificity of gene expression by the use of native promoters. To demonstrate how FTFLP may be used for characterization of the Arabidopsis proteome, we tagged a series of known proteins with diverse subcellular targeting patterns as well as several proteins with unknown function and unassigned subcellular localization.
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页码:25 / 38
页数:14
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