High-throughput fluorescent tagging of full-length arabidopsis gene products in planta

被引:189
作者
Tian, GW
Mohanty, A
Chary, SN
Li, SJ
Paap, B
Drakakaki, G
Kopec, CD
Li, JX
Ehrhardt, D
Jackson, D
Rhee, SY
Raikhel, NV
Citovsky, V
机构
[1] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA
[2] Watson Sch Biol Sci, Cold Spring Harbor, NY 11724 USA
[3] Univ Calif Riverside, Ctr Plant Cell Biol, Riverside, CA 92521 USA
[4] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA
[5] Arabidopsis Informat Resource, Stanford, CA 94305 USA
[6] Carnegie Inst Sci, Stanford, CA 94305 USA
[7] SUNY Stony Brook, Dept Biochem & Cell Biol, Stony Brook, NY 11794 USA
关键词
D O I
10.1104/pp.104.040139
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
We developed a high-throughput methodology, termed fluorescent tagging of full-length proteins (FTFLP), to analyze expression patterns and subcellular localization of Arabidopsis gene products in planta. Determination of these parameters is a logical first step in functional characterization of the approximately one-third of all known Arabidopsis genes that encode novel proteins of unknown function. Our FTFLP-based approach offers two significant advantages: first, it produces internally-tagged full-length proteins that are likely to exhibit native intracellular localization, and second, it yields information about the tissue specificity of gene expression by the use of native promoters. To demonstrate how FTFLP may be used for characterization of the Arabidopsis proteome, we tagged a series of known proteins with diverse subcellular targeting patterns as well as several proteins with unknown function and unassigned subcellular localization.
引用
收藏
页码:25 / 38
页数:14
相关论文
共 70 条
[1]   Strong, constitutive expression of the Arabidopsis ACT2/ACT8 actin subclass in vegetative tissues [J].
An, YQ ;
McDowell, JM ;
Huang, SR ;
McKinney, EC ;
Chambliss, S ;
Meagher, RB .
PLANT JOURNAL, 1996, 10 (01) :107-121
[2]   Analysis of the genome sequence of the flowering plant Arabidopsis thaliana [J].
Kaul, S ;
Koo, HL ;
Jenkins, J ;
Rizzo, M ;
Rooney, T ;
Tallon, LJ ;
Feldblyum, T ;
Nierman, W ;
Benito, MI ;
Lin, XY ;
Town, CD ;
Venter, JC ;
Fraser, CM ;
Tabata, S ;
Nakamura, Y ;
Kaneko, T ;
Sato, S ;
Asamizu, E ;
Kato, T ;
Kotani, H ;
Sasamoto, S ;
Ecker, JR ;
Theologis, A ;
Federspiel, NA ;
Palm, CJ ;
Osborne, BI ;
Shinn, P ;
Conway, AB ;
Vysotskaia, VS ;
Dewar, K ;
Conn, L ;
Lenz, CA ;
Kim, CJ ;
Hansen, NF ;
Liu, SX ;
Buehler, E ;
Altafi, H ;
Sakano, H ;
Dunn, P ;
Lam, B ;
Pham, PK ;
Chao, Q ;
Nguyen, M ;
Yu, GX ;
Chen, HM ;
Southwick, A ;
Lee, JM ;
Miranda, M ;
Toriumi, MJ ;
Davis, RW .
NATURE, 2000, 408 (6814) :796-815
[3]   Interactions of CcdB with DNA gyrase - Inactivation of GyrA, poisoning of the gyrase-DNA complex, and the antidote action of CcdA [J].
Bahassi, EM ;
O'Dea, MH ;
Allali, N ;
Messens, J ;
Gellert, M ;
Couturier, M .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (16) :10936-10944
[4]   Giving meaning to movement [J].
Barton, MK .
CELL, 2001, 107 (02) :129-132
[5]   Local, efflux-dependent auxin gradients as a common module for plant organ formation [J].
Benková, E ;
Michniewicz, M ;
Sauer, M ;
Teichmann, T ;
Seifertová, D ;
Jürgens, G ;
Friml, J .
CELL, 2003, 115 (05) :591-602
[6]   Function of microtubules in intercellular transport of plant virus RNA [J].
Boyko V. ;
Ferralli J. ;
Ashby J. ;
Schellenbaum P. ;
Heinlein M. .
Nature Cell Biology, 2000, 2 (11) :826-832
[7]   PROTEIN LIPIDATION IN CELL SIGNALING [J].
CASEY, PJ .
SCIENCE, 1995, 268 (5208) :221-225
[8]   GREEN FLUORESCENT PROTEIN AS A MARKER FOR GENE-EXPRESSION [J].
CHALFIE, M ;
TU, Y ;
EUSKIRCHEN, G ;
WARD, WW ;
PRASHER, DC .
SCIENCE, 1994, 263 (5148) :802-805
[9]   Structural elements required for the localization of ASH1 mRNA and of a green fluorescent protein reporter particle in vivo [J].
Chartrand, P ;
Meng, XH ;
Singer, RH ;
Long, RM .
CURRENT BIOLOGY, 1999, 9 (06) :333-336
[10]   Systemic transport of RNA in plants [J].
Citovsky, V ;
Zambryski, P .
TRENDS IN PLANT SCIENCE, 2000, 5 (02) :52-54