Development and Characterization of a Genotype 4 Hepatitis E Virus Cell Culture System Using a HE-JF5/15F Strain Recovered from a Fulminant Hepatitis Patient

被引：77

作者：

Tanaka, Toshinori ^{[1
]}

Takahashi, Masaharu ^{[1
]}

Takahashi, Hideyuki ^{[1
]}

Ichiyama, Koji ^{[1
]}

Hoshino, Yu ^{[1
]}

Nagashima, Shigeo ^{[1
]}

Mizuo, Hitoshi ^{[2
]}

Okamoto, Hiroaki ^{[1
]}

机构：

[1] Jichi Med Univ, Sch Med, Div Virol, Dept Infect & Immun, Shimotsuke, Tochigi 3290498, Japan

[2] Kin Ikyo Chuo Hosp, Dept Internal Med, Sapporo, Hokkaido, Japan

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2009年 / 47卷 / 06期

关键词：

A VIRUS; SPORADIC ACUTE; NUCLEOTIDE-SEQUENCE; IN-VIVO; JAPAN; GENOME; SWINE; INFECTION; HEV; TRANSMISSION;

D O I：

10.1128/JCM.00629-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We developed an efficient cell culture system for genotype 4 hepatitis E virus using the HE-JF5/15F strain recovered from a fulminant hepatitis patient. The sixth-passage virus in the culture supernatant reached 1.5 x 108 copies/ml at 10 days postinoculation and possessed 10 nucleotide mutations with four amino acid changes.

引用

页码：1906 / 1910

页数：5

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