High-throughput cellular microarray platforms: applications in drug discovery, toxicology and stem cell research

被引：207

作者：

Fernandes, Tiago G. ^{[1
,2
,5
]}

Diogo, Maria Margarida ^{[5
]}

Clark, Douglas S. ^{[4
]}

Dordick, Jonathan S. ^{[1
,2
,3
]}

Cabral, Joaquim M. S. ^{[5
]}

机构：

[1] Rensselaer Polytech Inst, Dept Chem & Biol Engn, Troy, NY 12180 USA

[2] Rensselaer Polytech Inst, Ctr Biotechnol & Interdisciplinary Studies, Troy, NY 12180 USA

[3] Rensselaer Polytech Inst, Dept Biol, Troy, NY 12180 USA

[4] Univ Calif Berkeley, Dept Chem Engn, Berkeley, CA 94720 USA

[5] Inst Super Tecn, Ctr Biol & Chem Engn, Inst Biotechnol & Bioengn IBB, P-1049001 Lisbon, Portugal

来源：

TRENDS IN BIOTECHNOLOGY | 2009年 / 27卷 / 06期

基金：

美国国家卫生研究院;

关键词：

GENE-FUNCTION; ON-CHIP; CULTURE; ARRAYS; MICROENVIRONMENTS; BIOMATERIALS; ASSAY; DIFFERENTIATION; TECHNOLOGIES; PROTEINS;

D O I：

10.1016/j.tibtech.2009.02.009

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Cellular microarrays are powerful experimental tools for high-throughput screening of large numbers of test samples. Miniaturization increases assay throughput while reducing reagent consumption and the number of cells required, making these systems attractive for a wide range of assays in drug discovery, toxicology, stem cell research and potentially therapy. Here, we provide an overview of the emerging technologies that can be used to generate cellular microarrays, and we highlight recent significant advances in the field. This emerging and multidisciplinary approach offers new opportunities for the design and control of stem cells in tissue engineering and cellular therapies and promises to expedite drug discovery in the biotechnology and pharmaceutical industries.

引用

页码：342 / 349

页数：8

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