Functional immobilization of signaling proteins enables control of stem cell fate

被引：146

作者：

Alberti, Kristin ^{[1
]}

Davey, Ryan E. ^{[2
]}

Onishi, Kento ^{[2
]}

George, Sophia ^{[3
]}

Salchert, Katrin ^{[1
]}

Seib, F. Philipp ^{[1
,4
]}

Bornhaeuser, Martin ^{[4
,5
]}

Pompe, Tilo ^{[1
]}

Nagy, Andras ^{[3
]}

Werner, Carsten ^{[1
,2
,5
]}

Zandstra, Peter W. ^{[2
,6
]}

机构：

[1] Max Bergmann Ctr Biomat, Leibniz Inst Polymer Res Dresden, D-01069 Dresden, Germany

[2] Univ Toronto, Inst Biomat & Biomed Engn, Toronto, ON M5S 3G9, Canada

[3] Mt Sinai Hosp, Samuel Lunenfeld Res Inst, Toronto, ON M5G 1X5, Canada

[4] Tech Univ Dresden, Univ Hosp Carl Gustav Carus, D-01307 Dresden, Germany

[5] Ctr Regenerat Therapies Dresden, D-01307 Dresden, Germany

[6] Univ Toronto, Toronto, ON M5S 3E5, Canada

来源：

NATURE METHODS | 2008年 / 5卷 / 07期

基金：

加拿大自然科学与工程研究理事会;

关键词：

D O I：

10.1038/nmeth.1222

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The mode of ligand presentation has a fundamental role in organizing cell fate throughout development. We report a rapid and simple approach for immobilizing signaling ligands to maleic anhydride copolymer thin-film coatings, enabling stable signaling ligand presentation at interfaces at defined concentrations. We demonstrate the utility of this platform technology using leukemia inhibitory factor (LIF) and stem cell factor (SCF). Immobilized LIF supported mouse embryonic stem cell (mESC) pluripotency for at least 2 weeks in the absence of added diffusible LIF. Immobilized LIF activated signal transducer and activator of transcription 3 (STAT3) and mitogen-activated protein kinase (MAPK) signaling in a dose-dependent manner. The introduced method allows for the robust investigation of cell fate responses from interface-immobilized ligands.

引用

页码：645 / 650

页数：6

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