Noninterferometric differential confocal microscopy with 2-nm depth resolution

被引:121
作者
Lee, CH [1 ]
Wang, JP [1 ]
机构
[1] NATL TAIWAN UNIV,DEPT ELECT ENGN,TAIPEI 10764,TAIWAN
关键词
D O I
10.1016/S0030-4018(96)00642-6
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
By utilizing the sharp slopes of the axial response curve of confocal imaging, we demonstrate a differential confocal technique for surface imaging with depth resolution as great as 2 nm. Neither servo feedback loop nor lock-in detection are used, hence measurements can be performed at high speed with long working distance. Because the technique uses no interferometric effects, it offers large open-loop dynamic range and is compatible with fluorescence microscopy.
引用
收藏
页码:233 / 237
页数:5
相关论文
共 9 条
[1]   IMAGING AND VIBRATIONAL ANALYSIS WITH LASER-FEEDBACK INTERFEROMETRY [J].
BEARDEN, A ;
ONEILL, MP ;
OSBORNE, LC ;
WONG, TL .
OPTICS LETTERS, 1993, 18 (03) :238-240
[2]  
BORN M, 1980, PRINCIPLES OPTICS, pCH8
[3]   SCANNING LASER MICROSCOPE [J].
DAVIDOVITS, P ;
EGGER, MD .
NATURE, 1969, 223 (5208) :831-+
[4]   2-PHOTON LASER SCANNING FLUORESCENCE MICROSCOPY [J].
DENK, W ;
STRICKLER, JH ;
WEBB, WW .
SCIENCE, 1990, 248 (4951) :73-76
[5]   COMPACT CONFOCAL INTERFERENCE MICROSCOPY [J].
JUSKAITIS, R ;
WILSON, T ;
REA, NP .
OPTICS COMMUNICATIONS, 1994, 109 (1-2) :167-177
[6]   STUDYING SINGLE LIVING CELLS AND CHROMOSOMES BY CONFOCAL RAMAN MICROSPECTROSCOPY [J].
PUPPELS, GJ ;
DEMUL, FFM ;
OTTO, C ;
GREVE, J ;
ROBERTNICOUD, M ;
ARNDTJOVIN, DJ ;
JOVIN, TM .
NATURE, 1990, 347 (6290) :301-303
[7]  
WEINSTEIN RS, 1974, RED BLOOD, V1, pCH5
[8]  
WIJNAENDTSVANRE.RW, 1985, J MICROSC-OXFORD, V138, P29
[9]  
WILSON T, 1990, CONFOCAL MICROSCOPY, pCH1