Distribution of 5-methylcytosine-rich regions in the metaphase chromosomes of Vicia faba

被引：25

作者：

Frediani, M

Giraldi, E

Castiglione, MR

机构：

[1] UNIV TUSCIA,DIPARTIMENTO AGROBIOL & AGROCHIM,I-01100 VITERBO,ITALY

[2] UNIV PISA,DIPARTIMENTO SCI BOT,I-56126 PISA,ITALY

来源：

CHROMOSOME RESEARCH | 1996年 / 4卷 / 02期

关键词：

DNA methylation; homologous chromosomes; Vicia faba;

D O I：

10.1007/BF02259707

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The DNA methylation pattern of Viola faba metaphase chromosomes was examined with a specific monoclonal antibody. 5-methylcytosine (5-mC) residues are present in different chromosomal sites, and are particularly abundant in telomeric and/or subtelomeric regions and in certain intercalary bands. Chromosomal localization of methylated regions enables a better knowledge of the lengthwise differentiation of this chromosome complement. Our results also indicate that there may be differences in monoclonal antibody binding between corresponding regions of homologous chromosomes in V. faba. This behaviour is detectable in specific regions with different frequencies. The data support results previously obtained for Allium cepa metaphase chromosomes using the same monoclonal antibody.

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页码：141 / 146

页数：6

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