SPARC stimulates the synthesis of OPG/OCIF, MMP-2 and DNA in human periodontal ligament cells

被引：21

作者：

Fujita, T ^{[1
]}

Shiba, H ^{[1
]}

Sakata, M ^{[1
]}

Uchida, Y ^{[1
]}

Nakamura, S ^{[1
]}

Kurihara, H ^{[1
]}

机构：

[1] Hiroshima Univ, Grad Sch Biomed Sci, Dept Periodontal Med, Div Frontier Med Sci,Minami Ku, Hiroshima 7348553, Japan

来源：

JOURNAL OF ORAL PATHOLOGY & MEDICINE | 2002年 / 31卷 / 06期

关键词：

ALPase activity; human periodontal ligament cells; MMP-2; OPG; OCIF; SPARC;

D O I：

10.1034/j.1600-0714.2002.00188.x

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Background: Osteonectin/secreted protein, acidic and rich in cysteine (SPARC) is expressed in periodontal ligaments. Therefore, a better understanding of the action of SPARC on periodontal ligament cells could help to elucidate remodelling and repair mechanisms in periodontal tissue. In the present study, we examined the effects of human platelet-derived SPARC (hp-SPARC) on the expressions of SPARC and osteoprotegerin/osteoclastogenesis inhibitory factor (OPG/OCIF), alkaline phosphatase (ALPase) activity, matrix metalloproteinase-2 (MMP-2) production and DNA synthesis in cultures of human periodontal ligament (HPL) cells. Methods: HPL cells at the sixth passage were exposed to hp-SPARC. The expression of OPG/OCIF and SPARC mRNAs was examined by Northern blot analysis. The protein levels for OPG/OCIF and MMP-2 were determined by Western blot analysis. ALPase activity was measured by the method of Bessey et al. DNA synthesis was estimated by incorporation of [(3) H]thymidine. Results: Hp-SPARC enhanced OPG/OCIF synthesis at the protein and mRNA levels. Hp-SPARC also enhanced DNA and MMP-2 synthesis dose-dependently, but had little effect on ALPase activity and SPARC mRNA expression. Conclusion: SPARC may play a role in remodelling and repair of periodontal tissue by promoting proliferation and MMP-2 production. It may also regulate osteoclast formation through OPG/OCIF in periodontal ligament cells.

引用

页码：345 / 352

页数：8

共 43 条

[31] EFFECTS OF BASIC FIBROBLAST GROWTH-FACTOR ON PROLIFERATION, THE EXPRESSION OF OSTEONECTIN (SPARC) AND ALKALINE-PHOSPHATASE, AND CALCIFICATION IN CULTURES OF HUMAN PULP CELLS [J].