Distribution of RuBisCO genotypes along a redox gradient in Mono Lake, California

被引:59
作者
Giri, BJ [1 ]
Bano, N [1 ]
Hollibaugh, JT [1 ]
机构
[1] Univ Georgia, Dept Marine Sci, Athens, GA 30602 USA
关键词
D O I
10.1128/AEM.70.6.3443-3448.2004
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Partial sequences of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) (EC 4.1.1.39) genes were retrieved from samples taken along a redox gradient in alkaline, hypersaline Mono Lake, Calif. The form I gene (cbbL) was found in all samples, whereas form H (cbbM) was not retrieved from any of the samples. None of the RuBisCO sequences we obtained were closely related (nucleotide similarity, <90%) to sequences in the database. Some could be attributed to organisms isolated from the lake (Cyanobium) or appearing in enrichment cultures. Most (52%) of the sequences fell into in one clade, containing sequences that were identical to sequences retrieved from an enrichment culture grown with nitrate and sulfide, and another clade contained sequences identical to those retrieved from an arsenate-reducing, sulfide-oxidizing enrichment.
引用
收藏
页码:3443 / 3448
页数:6
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