Improved capillary electrophoretic separation of nine (fluoro)quinolones with fluorescence detection for biological and environmental samples

被引：108

作者：

Ferdig, M ^{[1
]}

Kaleta, A ^{[1
]}

Vo, TDT ^{[1
]}

Buchberger, W ^{[1
]}

机构：

[1] Johannes Kepler Univ Linz, Inst Analyt Chem, A-4040 Linz, Austria

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1047卷 / 02期

关键词：

fluoroquinolones; quinolones; antibiotics;

D O I：

10.1016/j.chroma.2004.07.005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A capillary electrophoretic method has been developed for the separation of nine (fluoro)quinolones. Detection is done by fluorescence measurement with broad wavelength band excitation between 240 and 400 nm. Best separation is achieved in a carrier electrolyte containing 50 mM H3PO4 adjusted to pH 7.55-acetonitrile (60:40, v/v). Detection limits are in the low mug l(-1) range. The suitability to real samples has been demonstrated by analyzing blood samples and surface water samples. Sample preconcentration and sample clean-up can easily be done by solid-phase extraction. Different phases based on alkyl- or phenyl-modified silica as well as on polymers have been investigated for this purpose. The method should also be useful for determination of residues of (fluoro)quinolones in food or other matrices. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：305 / 311

页数：7

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