A novel group of oleosins is present inside the pollen of Arabidopsis

被引:145
作者
Kim, HU [1 ]
Hsieh, K [1 ]
Ratnayake, C [1 ]
Huang, AHC [1 ]
机构
[1] Univ Calif Riverside, Dept Bot & Plant Sci, Ctr Plant Cell Biol, Riverside, CA 92521 USA
关键词
D O I
10.1074/jbc.M109298200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In plants, subcellular triacylglycerol granules in seeds (oil bodies) and floral tapetum (tapetosomes) are stabilized by amphipathic structural protein called oleosin. We hereby report a novel group of oleosins that is present inside the pollen of Arabidopsis thaliana. We have used the conserved sequence of oleosins to locate, via the DNA database, all 16 oleosin genes in the Arabidopsis genome. The oleosin genes can be divided into three groups according to their sequences and tissue-specific expressions, as probed by RNA blot hybridization and reverse transcriptase-PCR. The first group includes eight genes specifically expressed in the floret tapetum. The second group includes five genes specifically expressed in maturing seeds. The third, novel group includes three genes expressed in both maturing seeds and floral microspores, which will become pollen. Transgenic study using the promoter of one of these genes attached to a reporter gene has provided corroborative evidence for the specific expression of the gene in the microspores in the florets. One of the pollen oleosins can be identified by microsequencing and specific immunoblotting. Pollen oleosins synthesized by recombinant bacteria can collaborate with phospholipids in stabilizing reconstituted oil bodies. Thus, pollen has oleosins to stabilize the abundant subcellular oil bodies. Seed oil bodies and floret tapetosomes have been isolated from the miniature Arabidopsis plants, and the success indicates that the organelles can be subjected to future biochemical and genetic studies.
引用
收藏
页码:22677 / 22684
页数:8
相关论文
共 40 条
[1]  
Bowman J., 1994, ARABIDOPSIS ATLAS MO
[2]   Floral dip:: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana [J].
Clough, SJ ;
Bent, AF .
PLANT JOURNAL, 1998, 16 (06) :735-743
[3]  
Davis R.A., 1996, BIOCH LIPIDS LIPOPRO, P473
[4]   DIFFERENTIAL EXPRESSION OF 5 ARABIDOPSIS GENES ENCODING GLYCINE-RICH PROTEINS [J].
DEOLIVEIRA, DE ;
SEURINCK, J ;
INZE, D ;
VANMONTAGU, M ;
BOTTERMAN, J .
PLANT CELL, 1990, 2 (05) :427-436
[5]  
DEOLIVEIRA DE, 1993, PLANT J, V3, P495, DOI 10.1046/j.1365-313X.1993.03040495.x
[6]   FIBRIL ASSEMBLY AND CAROTENOID OVERACCUMULATION IN CHROMOPLASTS - A MODEL FOR SUPRAMOLECULAR LIPOPROTEIN STRUCTURES [J].
DERUERE, J ;
ROMER, S ;
DHARLINGUE, A ;
BACKHAUS, RA ;
KUNTZ, M ;
CAMARA, B .
PLANT CELL, 1994, 6 (01) :119-133
[7]   Re-evaluation of the primary structure of Ralstonia eutropha phasin and implications for polyhydroxyalkanoic acid granule binding [J].
Hanley, SZ ;
Pappin, DJC ;
Rahman, D ;
White, AJ ;
Elborough, KM ;
Slabas, AR .
FEBS LETTERS, 1999, 447 (01) :99-105
[8]   Adipocyte differentiation-related protein is secreted into milk as a constituent of milk lipid globule membrane [J].
Heid, HW ;
Schnolzer, M ;
Keenan, TW .
BIOCHEMICAL JOURNAL, 1996, 320 :1025-1030
[9]   OIL BODIES AND OLEOSINS IN SEEDS [J].
HUANG, AHC .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1992, 43 :177-200
[10]  
JEFFERSON RA, 1987, EMBO J, V6, P3901