DNA sensor for base mismatch detection based on a gold colloid modified glassy carbon electrode

被引：5

作者：

Lin, XQ ^{[1
]}

Zheng, SJ

Miao, Q

Jin, BK

机构：

[1] Univ Sci & Technol China, Dept Chem, Hefei 230026, Anhui, Peoples R China

[2] Wenzhou Normal Univ, Dept Chem, Wenzhou 325000, Zhejiang, Peoples R China

[3] Anhui Univ, Dept Chem, Hefei 230000, Anhui, Peoples R China

来源：

ANALYTICAL LETTERS | 2002年 / 35卷 / 08期

关键词：

DNA sensor; gold colloid; 2-NH2(CH2)(2)SH; cyclic voltammetry;

D O I：

10.1081/AL-120006673

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An ss-DNA/NG/AET/GCE electrochemical sensor was prepared with synthesized 15 mer ss-DNA probes. The electrode potential for immobilization of the probes, the electrode potential for hybridization reaction, and the reaction time for hybridization of the DNA sensor were carefully studied as significant factors affecting the sensitivity and selectivity of hybridization detection. The primary study showed that the sensor could discriminate well the 5 bases mismatch, 3 bases mismatch and 1 base mismatch at optimized conditions.

引用

页码：1373 / 1385

页数：13

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