The Epstein-Barr virus nuclear antigen-1 may act as a transforming suppressor of the HER2/neu oncogene

被引：11

作者：

Chuang, TC

Way, TD

Lin, YS

Lee, YC

Law, SL

Kao, MC ^{[1
]}

机构：

[1] Natl Def Univ, Grad Inst Life Sci, Natl Def Med Ctr, Taipei, Taiwan

[2] Vet Gen Hosp, Dept Med Res & Educ, Pharmaceut Res Lab, Taipei 112, Taiwan

[3] Natl Def Univ, Dept Biochem, Natl Def Med Ctr, Taipei, Taiwan

来源：

FEBS LETTERS | 2002年 / 532卷 / 1-2期

关键词：

HER2/neu; EBNA1; tumorigenicity; ovarian cancer;

D O I：

10.1016/S0014-5793(02)03658-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

It is known that the HER2/neu proto-oncogene is associated with a wide variety of human cancers and considered to be an attractive target for developing anti-cancer agents. We report here for the first time that the Epstein-Barr virus nuclear antigen-1 (EBNA1) suppresses the HER2/neu oncogene expression at the transcriptional level. Recombinant clones of EBNA1 were subcloned and stably transfected into HER2/neu-over-expressing human ovarian cancer SKOV3.ip1 cells. These EBNA1-containing clones down-regulated the endogenous production of P185(HER2/neu). In addition, the EBNA1-expressing stable transfectants showed reduced growth rate, low soft agarose colony-forming ability and tumorigenic potential as compared with the parental line. These data suggest that EBNA1 may act as a transforming suppressor of the HER2/neu oncogene. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：135 / 142

页数：8

共 34 条

[1] FUNCTIONAL DOMAINS OF EPSTEIN-BARR-VIRUS NUCLEAR ANTIGEN EBNA-1 [J].