Klumpfuss, a putative Drosophila zinc finger transcription factor, acts to differentiate between the identities of two secondary precursor cells within one neuroblast lineage

被引:41
作者
Yang, XH [1 ]
Bahri, S [1 ]
Klein, T [1 ]
Chia, W [1 ]
机构
[1] NATL UNIV SINGAPORE, INST MOL & CELL BIOL, SINGAPORE 119260, SINGAPORE
关键词
Drosophila embryonic CNS; neuronal diversity; neuroblast lineage; cell fate specification;
D O I
10.1101/gad.11.11.1396
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The similar to 300 distinct neurons comprising each hemineuromere of the Drosophila embryonic central nervous system are derived from a segmentally reiterated array of similar to 30 progenitor sells, neuroblasts (NBs). Each NB has a unique identity and undergoes repeated cell divisions to product several smaller secondary precursor cells, ganglion mother cells (GMCs); each GMC divides once to produce two neurons and/or glia, thereby generating a specific lineage of neurons/glia. Understanding the generation of neuronal diversity requires not only elucidation of the molecules and mechanisms that specify NB identity but also those that act to differentiate between the cell types produced within one NB lineage. Here we show that the Drosophila Zn finger protein Klumpfuss (Klu), which shows sequence similarities to the mammalian Wilm's tumor suppressor (WT-1), acts to differentiate between the identities of the first two secondary precursor cells produced front one NB lineage. Klu is expressed in the NB4-2 Lineage only after two rounds of NB cell division, in the second born GMC (GMC4-2b). In loss-of-function mutant embryos, the first born GMC (GMC4-2a) as well as its progeny neurons are duplicated; we show that this duplication of the GMC4-2a sublineage arises because GMC4-2b adopts the identity of GMC4-2a and divides to produce the GMC4-2a progeny. Moreover, when Klu is ectopically expressed in GMC4-2a, it fails to acquire its normal identity and fails to produce correctly specified progeny. klu therefore acts to specify the identity of GMC4-2b and to make it distinct from GMC4-2a. Our findings further suggest that the determination of GMC cell fate occurs in two steps; the initial GMC identity is the consequence of inheritance from the maternal NB, however, the subsequent stabilization of this identity requires functions like klu in the GMC.
引用
收藏
页码:1396 / 1408
页数:13
相关论文
共 51 条
[11]  
Campos-Ortega Jose A., 1993, P1091
[12]   HUCKEBEIN SPECIFIES ASPECTS OF CNS PRECURSOR IDENTITY REQUIRED FOR MOTONEURON AXON PATHFINDING [J].
CHULAGRAFF, Q ;
SCHMID, A ;
LEIDEL, J ;
BRONNER, G ;
JACKLE, H ;
DOE, CQ .
NEURON, 1995, 15 (05) :1041-1051
[13]   NEUROBLAST SPECIFICATION AND FORMATION REGULATED BY WINGLESS IN THE DROSOPHILA CNS [J].
CHULAGRAFF, Q ;
DOE, CQ .
SCIENCE, 1993, 261 (5128) :1594-1597
[14]  
CUI X, 1992, DEVELOPMENT, V116, P943
[15]   2 CLOSELY LINKED DROSOPHILA POU DOMAIN GENES ARE EXPRESSED IN NEUROBLASTS AND SENSORY ELEMENTS [J].
DICK, T ;
YANG, XH ;
YEO, SL ;
CHIA, W .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (17) :7645-7649
[16]   THE PROSPERO GENE SPECIFIES CELL FATES IN THE DROSOPHILA CENTRAL-NERVOUS-SYSTEM [J].
DOE, CQ ;
CHULAGRAFF, Q ;
WRIGHT, DM ;
SCOTT, MP .
CELL, 1991, 65 (03) :451-464
[17]   CONTROL OF NEURONAL FATE BY THE DROSOPHILA SEGMENTATION GENE EVEN-SKIPPED [J].
DOE, CQ ;
SMOUSE, D ;
GOODMAN, CS .
NATURE, 1988, 333 (6171) :376-378
[18]   EXPRESSION AND FUNCTION OF THE SEGMENTATION GENE FUSHI-TARAZU DURING DROSOPHILA NEUROGENESIS [J].
DOE, CQ ;
HIROMI, Y ;
GEHRING, WJ ;
GOODMAN, CS .
SCIENCE, 1988, 239 (4836) :170-175
[19]  
DOE CQ, 1992, DEVELOPMENT, V116, P855
[20]   IDENTIFICATION AND CELL LINEAGE OF INDIVIDUAL NEURAL PRECURSORS IN THE DROSOPHILA CNS [J].
DOE, CQ ;
TECHNAU, GM .
TRENDS IN NEUROSCIENCES, 1993, 16 (12) :510-514