SARS antibody test for serosurveillance

被引：22

作者：

Hsueh, PR

Kao, CL

Lee, CN

Chen, LK

Ho, MS

Sia, C

De Fang, X

Lynn, S

Chang, TY

Liu, SK

Walfield, AM

Wang, CY

机构：

[1] United Biomed Inc, Hauppauge, NY 11746 USA

[2] Natl Taiwan Univ, Coll Med, Taipei, Taiwan

[3] Tsu Chi Univ, Hualien, Taiwan

[4] Acad Sinica, Taipei 115, Taiwan

[5] United Biomed Inc, Hsinchu, Taiwan

来源：

EMERGING INFECTIOUS DISEASES | 2004年 / 10卷 / 09期

关键词：

D O I：

10.3201/eid1009.040101

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A peptide-based enzyme-linked immunosorbent assay (ELISA) can be used for retrospective serosurveillance of severe acute respiratory syndrome (SARS) by helping identify undetected chains of disease transmission. The assay was developed by epitope mapping, using synthetic peptides from the spike, membrane, and nucleocapsid protein sequences of SARS-associated coronavirus. The new peptide ELISA consistently detected seroconversion by week 2 of onset of fever, and seropositivity remained through day 100. Specificity was 100% on normal blood donor samples, on serum samples associated with infection by other pathogens, and on an interference panel. The peptide-based test has advantages of safety, standardization, and automation over previous immunoassays for SARS. The assay was used for a retrospective survey of healthy healthcare workers in Taiwan who treated SARS patients. Asymptomatic seroconversions were detected in two hospitals that had nosocomial disease.

引用

收藏

页码：1558 / 1562

页数：5

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