Cellular expression of GDNF mRNA suggests multiple functions inside and outside the nervous system

被引:199
作者
Nosrat, CA
Tomac, A
Lindqvist, E
Lindskog, S
Humpel, C
Stromberg, I
Ebendal, T
Hoffer, BJ
Olson, L
机构
[1] KAROLINSKA INST, DEPT NEUROSCI, S-17177 STOCKHOLM, SWEDEN
[2] KAROLINSKA INST, HUDDINGE HOSP, DEPT ORAL DIAG, DEPT ORAL PATHOL, S-14104 HUDDINGE, SWEDEN
[3] UNIV INNSBRUCK, DEPT PSYCHIAT, NEUROCHEM UNIT, A-6020 INNSBRUCK, AUSTRIA
[4] UNIV UPPSALA, CTR BIOMED, DEPT DEV NEUROSCI, S-75123 UPPSALA, SWEDEN
[5] UNIV COLORADO, HLTH SCI CTR, DEPT PHARMACOL, DENVER, CO 80262 USA
关键词
glial-cell-line-derived neurotrophic factor; in situ hybridization; prenatal and postnatal development; basal ganglia; kidney; tooth development; gastrointestinal tract; rat (Sprague Dawley); pig;
D O I
10.1007/s004410050688
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Glial-cell-line-derived neurotrophic factor (GDNF) is a distant member of the transforming growth factor-beta family and has potent neurotrophic effects on several classes of neurons including dopamine neurons and motoneurons. Here, we have used in situ hybridization to describe the development of the cellular expression of GDNF mRNA pre- and postnatally. Consistent with dopaminotrophic activity, GDNF mRNA is expressed in the developing basal ganglia and the olfactory tubercle. It is also found in a thalamic nucleus, in neurons of the substantia innominata, in the developing Purkinje neurons and the developing locus coeruleus area, and in trigeminal brainstem nuclei. In the spinal cord, neuronal expression is found in Clarke's column. GDNF mRNA is also expressed in the dorsal horns during development. Additional GDNF mRNA expression in the head region includes the carotid body, the retina, the vibrissae, the inner ear, the ear canal, and epithelium in the nasal cavity. Prominent expression is also found in the developing teeth. The widespread expression of GDNF in developing skeletal muscle is consistent with trophic activity on a-motoneurons. The smooth muscle layers of the gastrointestinal tract are also strongly positive. A very strong signal is found in the outer mesenchyme of the developing metanephric kidney. We conclude that GDNF mRNA is expressed in many different cellular systems inside and outside the central nervous system during development, suggesting multiple functions of GDNF in the developing organism.
引用
收藏
页码:191 / 207
页数:17
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