Methylation sequencing from limiting DNA: embryonic, fixed, and microdissected cells

被引：40

作者：

Millar, DS

Warnecke, PM

Melki, JR

Clark, SJ

机构：

[1] Royal Prince Alfred Hosp, Kanematsu Labs, Sydney Canc Ctr, Camperdown, NSW 2050, Australia

[2] CSIRO, Div Mol Sci, Sydney Lab, N Ryde, NSW 1670, Australia

[3] Univ Sydney, Fac Med, Sydney, NSW 2050, Australia

来源：

METHODS | 2002年 / 27卷 / 02期

关键词：

bisulfite; embryo; DNA methylation; laser captured; microdissection; paraffin;

D O I：

10.1016/S1046-2023(02)00061-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

It is frequently useful to determine the methylation state of samples containing limited amounts of DNA such as from embryos, or from fixed tissue samples in which DNA is degraded or difficult to isolate. By modification of the standard protocols for DNA preparation and bisulfite treatment, it is possible to obtain DNA methylation sequence data for such samples. We present methods for bisulfite treatment of embryos, fixed sections, and samples obtained by laser capture microdissection, and discuss the additional experimental considerations required when working with small numbers of cells or degraded DNA samples. (C) 2002 Published by Elsevier Science (USA).

引用

页码：108 / 113

页数：6

共 24 条

[1] Sodium bisulfite analysis of the methylation status of DNA from small portions of paraffin slides [J].