Study of the mechanism of diatom cell division by means of 29Si isotope tracing

被引：11

作者：

Audinot, J. -N. ^{[1
]}

Guignard, C. ^{[1
]}

Migeon, H. -N. ^{[1
]}

Hoffmann, L. ^{[1
]}

机构：

[1] Ctr Rech Publ Gabriel Lippmann, Lab Anal Mat, L-4422 Belvaux, Luxembourg

来源：

APPLIED SURFACE SCIENCE | 2006年 / 252卷 / 19期

关键词：

NanoSIMS; isotope tracing; diatoms; biological sample; silicon;

D O I：

10.1016/j.apsusc.2006.02.115

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Diatoms are delicate unicellular organisms enclosed in a silica frustule, that is made up of two valves. Multiplication of the diatoms occurs by ordinary mitotic cell division. During cell division each cell produces two daughter cells, each of them keeping one of the two valves of the mother cell and producing a new valve by absorbing the silicon present in the environment. The NanoSIMS 50 allows ion imaging to be performed on diatoms in order to determine the site of fixation of silicon. The aim of this study was to observe and compare the mechanism of the construction of the new valve after cell division. To this end, different types of diatoms have been transferred in a culture medium enriched with Si-29 and after several days, the distribution of the different isotopes of silicon has been determined by NanoSIMS50 imaging. The construction of new valves has been observed and the isotopic ratio has been determined. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：6813 / 6815

页数：3

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