Progress in analytical imaging of the cell by dynamic secondary ion mass spectrometry (SIMS microscopy)

被引：197

作者：

Guerquin-Kern, JL ^{[1
]}

Wu, TD ^{[1
]}

Quintana, C ^{[1
]}

Croisy, A ^{[1
]}

机构：

[1] Ctr Univ Orsay, Lab Microscopie Inon, Inst Curie Rech, F-91405 Orsay, France

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 2005年 / 1724卷 / 03期

基金：

澳大利亚研究理事会;

关键词：

ultrastructure; cell imaging; ion microscopy; dynamic secondary ion mass spectrometry; parallel detection; stable isotope;

D O I：

10.1016/j.bbagen.2005.05.013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper reviews the most recent methodological advances in the field of biological imaging using dynamic secondary ion mass spectrometry (SIMS). After a short reminder of the basic principle of SIMS imaging, the latest high-resolution dynamic SIMS equipment is briefly described. This new ion nanoprobe (CAMECA NanoSIMS 50 (TM)) has a lateral resolution of less than 50 nm with primary Cs+ ion, the ability to detect simultaneously 5 different ions from the same micro-volume and a very good transmission even at high mass resolution (60% at M/Delta M=5000). Basic considerations related to sample preparation, mass resolution and primary ion implantation are given. The decisive capability of this new instrument, and more generally of high-resolution dynamic SIMS imaging in biology, are illustrated with the most recent examples of utilization(2). (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：228 / 238

页数：11

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