A jet impingement investigation of osteoblastic cell adhesion

被引:15
作者
Giliberti, DC [1 ]
Anderson, KA [1 ]
Dee, KC [1 ]
机构
[1] Tulane Univ, Dept Biomed Engn, New Orleans, LA 70118 USA
来源
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH | 2002年 / 62卷 / 03期
关键词
bone marrow stromal cells; cell adhesion; integrins; jet impingement; peptide-modified surfaces;
D O I
10.1002/jbm.10343
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
When designing dental and orthopedic implants, it is important to consider phenomena occurring at the microscopic level, particularly at the bone-implant interface. The presence of hard tissue at this interface is essential to implant viability. The integrity of this tissue-biomaterial interface is dependent on appropriate osteoblast functions (adhesion, matrix deposition, etc.) in the immediate area. Researchers have modified various materials with cell-adhesive peptides with the ultimate goal of controlling osteoblast functions. This study used microjet impingement to compare the strength of adhesion of osteoblastic cells (at varying populations) and fibroblasts to peptide-modified substrates m the presence and absence of fetal bovine serum. In the presence of the serum, there was no significant difference in cellular adhesion strength between substrates. In the absence of serum, all cells tested adhered more strongly to underlying substrates, and the strength of cellular adhesion was greater on modified surfaces than on plain glass surfaces. In the absence of serum, second-passage osteoblastic cells generally adhered to substrates more strongly than first-passage osteoblastic cells; fibroblasts adhered similarly to second-passage osteoblastic cells. Fundamental studies such as the present increase the understanding of cell adhesion to various substrates-knowledge that may be ultimately useful in creating an optimal bone-implant interface. 2002 (C) Wiley Periodicals, Inc.
引用
收藏
页码:422 / 429
页数:8
相关论文
共 83 条
[21]   Effects of roughness, fibronectin and vitronectin on attachment, spreading, and proliferation of human osteoblast-like cells (Saos-2) on titanium surfaces [J].
Degasne, I ;
Baslé, MF ;
Demais, V ;
Huré, G ;
Lesourd, M ;
Grolleau, B ;
Mercier, L ;
Chappard, D .
CALCIFIED TISSUE INTERNATIONAL, 1999, 64 (06) :499-507
[22]   SUBMERGED LAMINAR JET IMPINGEMENT ON A PLANE [J].
DESHPANDE, MD ;
VAISHNAV, RN .
JOURNAL OF FLUID MECHANICS, 1982, 114 (JAN) :213-236
[23]   Effect of serum proteins on osteoblast adhesion to surface-modified bioactive glass and hydroxyapatite [J].
El-Ghannam, A ;
Ducheyne, P ;
Shapiro, IM .
JOURNAL OF ORTHOPAEDIC RESEARCH, 1999, 17 (03) :340-345
[24]  
Fowkes FM., 1964, CONTACT ANGLE WETTAB, V43, P1, DOI DOI 10.1021/BA-1964-0043.CH001
[25]   Quantification of cell adhesion using a spinning disc device and application to surface-reactive materials [J].
Garcia, AJ ;
Ducheyne, P ;
Boettiger, D .
BIOMATERIALS, 1997, 18 (16) :1091-1098
[26]  
García AJ, 1998, J BIOMED MATER RES, V40, P48, DOI 10.1002/(SICI)1097-4636(199804)40:1<48::AID-JBM6>3.0.CO
[27]  
2-R
[28]  
Garratt AN, 1995, ACTA ANAT, V154, P34
[29]  
Göpferich A, 1999, J BIOMED MATER RES, V46, P390
[30]   Integrin-mediated interactions between human bone marrow stromal precursor cells and the extracellular matrix [J].
Gronthos, S ;
Simmons, PJ ;
Graves, SE ;
Robey, PG .
BONE, 2001, 28 (02) :174-181