Stimulation of brook trout ovarian steroidogenesis by gonadotropins I and II is mediated by the cyclic adenosine 3',5'-monophosphate protein kinase A pathway

被引:46
作者
Planas, JV
Goetz, FW
Swanson, P
机构
[1] UNIV WASHINGTON, SCH FISHERIES, SEATTLE, WA 98195 USA
[2] UNIV NOTRE DAME, DEPT BIOL SCI, NOTRE DAME, IN 46556 USA
[3] NOAA, NATL MARINE FISHERIES SERV, NW FISHERIES SCI CTR, SEATTLE, WA 98112 USA
关键词
D O I
10.1095/biolreprod57.3.647
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
In teleosts, ovarian steroidogenesis is under the control of two gonadotropic hormones, GTH I and GTH II, that are structurally and functionally homologous to FSH and LH. The intracellular mechanisms by which GTH I and GTH II stimulate steroidogenesis in the teleost ovary are not well understood. The purpose of this study was to investigate the involvement of the cAMP/protein kinase A (PKA) and protein kinase C (PKC)/Ca2+ signaling pathways in the steroidogenic actions of GTH I and GTH II in the ovary of the brook trout (Salvelinus fontinalis). The cAMP/PKA pathway mediated the actions of GTH I before germinal vesicle breakdown (preGVBD) and GTH II after GVBD and before ovulation (preOV). Experimental increases in intracellular cAMP concentration mimicked the steroidogenic effects of GTH I and GTH II, and an antagonistic analog of cAMP partially blocked them. In addition, GTH I and GTH II stimulated the production of cAMP in preGVBD and preOV follicles, respectively. Activation of the PKC/Ca2+ pathway by a phorbol ester or a Ca2+ ionophore blocked the GTH I-and GTH II-induced steroid production, whereas inhibition of PKC by specific inhibitors potentiated the effects of GTH I. These results suggest that the cAMP/PKA signaling pathway mediates the stimulatory effects of GTH I and GTH II on steroidogenesis, and they also suggest the additional involvement of the PKC/Ca2+ signaling pathway in modulating the actions of gonadotropins in brook trout ovarian follicles.
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页码:647 / 654
页数:8
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