The effects of hydrostatic pressure change on DNA integrity in the hydrothermal-vent mussel Bathymodiolus azoricus:: implications for future deep-sea mutagenicity studies

被引：42

作者：

Dixon, DR

Pruski, AM

Dixon, LRJ

机构：

[1] Southampton Oceanog Ctr, Southampton SO14 3ZH, Hants, England

[2] Observ Oceanol Banyuls, Lab Arago, F-66651 Banyuls Sur Mer, France

[3] Marine Biol Assoc UK, Plymouth PL1 2PB, Devon, England

来源：

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 2004年 / 552卷 / 1-2期

关键词：

hydrostatic pressure; deep-sea vents; comet assay; agarose gel electrophoresis assay; Bathymodiolus azoricus; environmental mutagens;

D O I：

10.1016/j.mrfmmm.2004.06.026

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Comet and agarose gel electrophoresis (AGE) assays were used to show that haemocytes (blood cells) and gill tissues of vent mussels, Bathymodiolus azoricus, are sensitive to hydrostatic pressure change, but can repair DNA damage induced by retrieval from 840 m to the sea surface. In contrast, animals collected from 1700 m survived for only a few days in the laboratory, which was reflected in their poor DNA quality. These findings support the hypothesis of a physiological barrier to survival around 1000-1500m depth, which these results show affects both vent and non-vent species alike. Based on in vitro experimental exposures to hydrogen peroxide and MMC, vent mussels appear to have sensitivities to the environmental mutagens that are not significantly different from those of coastal mussels. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：235 / 246

页数：12

共 31 条

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