Initial Clonal Expansion of Germinal Center B Cells Takes Place at the Perimeter of Follicles

被引:103
作者
Coffey, Francis [1 ]
Alabyev, Boris [1 ]
Manser, Tim [1 ]
机构
[1] Thomas Jefferson Univ, Dept Microbiol & Immunol, Philadelphia, PA 19107 USA
基金
美国国家卫生研究院;
关键词
PRIMARY IMMUNE-RESPONSE; IN-SITU; (4-HYDROXY-3-NITROPHENYL)ACETYL; ACTIVATION; AFFINITY; ANTIGEN; REGION; VISUALIZATION; EXPRESSION; MIGRATION;
D O I
10.1016/j.immuni.2009.01.011
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Current models of the germinal center (GC) response propose that after stimulation at the edges of T cell zones, pre-GC B cells directly migrate to the center of follicles and proliferate to form GCs. We followed the interrelationship of proliferation, differentiation, and microenvironmental locale in populations of pre-GC B cells responding to antigen. In contrast to the predictions of current models, after accumulation at the T-B interface, these cells appeared at the perimeter of follicles adjacent to the marginal zone. There, they rapidly proliferated for several days but underwent no V gene hypermutation and little heavy-chain class switching. Their chemokine receptor expression pattern indicated that these cells were sessile, yet they had begun to acquire many phenotypic characteristics of GC B cells. The expanded clones were subsequently observed in the center of follicles, suggesting that GCs are created by coalescence of B cells from this follicular perimeter response.
引用
收藏
页码:599 / 609
页数:11
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