Influence of stock plant pretreatment on gynogenic embryo induction from flower buds of onion

被引：23

作者：

Puddephat, IJ ^{[1
]}

Robinson, HT ^{[1
]}

Smith, BM ^{[1
]}

Lynn, J ^{[1
]}

机构：

[1] Hort Res Int, Wellesbourne CV35 9EF, Warwick, England

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 1999年 / 57卷 / 02期

关键词：

Allium cepa L; double haploid; gynogenesis; stock-plant-pre-treatment;

D O I：

10.1023/A:1006312614874

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The gynogenic response of a range of onion genotypes to flower bud culture was compared using a two-step culture system. Embryogenic cultures and plantlets were produced from unpollinated ovules in whole flower bud explants 6 to 19 weeks after culture initiation. Preconditioning stock plants significantly influenced gynogenic embryogenesis. A ten-fold increase in embryogenesis was obtained when flower buds were cultured from stock plants maintained at 15 degrees C compared to 10 degrees C or the ambient temperature conditions of a glasshouse (maximum-minimum of 25-12.7 degrees C). A total of 49 embryos was obtained from 2660 cultured flower buds and 45% of plantlets were successfully acclimatised to glasshouse conditions. The majority of acclimatised plantlets were haploid (68%) but spontaneous double haploid plants (23%) were obtained from three genotypes.

引用

页码：145 / 148

页数：4

共 15 条

[1] STUDIES OF GYNOGENESIS IN ONION (ALLIUM-CEPA L) - INDUCTION PROCEDURES AND GENETIC-ANALYSIS OF REGENERANTS [J].

BOHANEC, B ;

JAKSE, M ;

IHAN, A ;

JAVORNIK, B .

PLANT SCIENCE, 1995, 104 (02) :215-224

[2]

BOHANEC B, 1995, P 1995 NAT ON RES C, P23

[3] ADVANCES IN HAPLOID PLANT INDUCTION IN ONION (ALLIUM-CEPA L) THROUGH INVITRO GYNOGENESIS [J].

CAMPION, B ;

AZZIMONTI, MT ;

VICINI, E ;

SCHIAVI, M ;

FALAVIGNA, A .

PLANT SCIENCE, 1992, 86 (01) :97-104

[4] INDUCTION OF HAPLOID PLANTS IN ONION (ALLIUM-CEPA L) BY INVITRO CULTURE OF UNPOLLINATED OVULES [J].

CAMPION, B ;

ALLONI, C .

PLANT CELL TISSUE AND ORGAN CULTURE, 1990, 20 (01) :1-6

[5] GYNOGENIC LINES OF ONION (ALLIUM-CEPA L) - EVIDENCE OF THEIR HOMOZYGOSITY [J].

CAMPION, B ;

BOHANEC, B ;

JAVORNIK, B .

THEORETICAL AND APPLIED GENETICS, 1995, 91 (04) :598-602

[6] IMPROVED GROWTH OF TISSUE-CULTURES OF ONION, ALLIUM-CEPA [J].

DUNSTAN, DI ;

SHORT, KC .

PHYSIOLOGIA PLANTARUM, 1977, 41 (01) :70-72

[7]

GAMBORG OL, 1968, EXP CELL RES, V50, P112

[8] Variation of gynogenesis ability in onion (Allium cepa L) [J].

Geoffriau, E ;

Kahane, R ;

Rancillac, M .

EUPHYTICA, 1997, 94 (01) :37-44

[9]

Jakse M, 1996, PLANT CELL REP, V15, P934, DOI 10.1007/BF00231591

[10]

KELLER J, 1990, EUPHYTICA, V47, P241

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