Live-cell imaging with EosFP and other photoactivatable marker proteins of the GFP family

被引：66

作者：

Wiedenmann, Joerg

Nienhaus, G. Ulrich ^{[1
]}

机构：

[1] Univ Ulm, Dept Biophys, D-89069 Ulm, Germany

[2] Univ Ulm, Dept Gen Zool & Endocrinol, D-89069 Ulm, Germany

[3] Univ Illinois, Dept Phys, Urbana, IL 61801 USA

来源：

EXPERT REVIEW OF PROTEOMICS | 2006年 / 3卷 / 03期

关键词：

anthozoa; crystal structure; EosFP; green fluorescent proteins; photoactivation; photoconversion; protein tracking; red fluorescent protein;

D O I：

10.1586/14789450.3.3.361

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Fluorescent proteins from the green fluorescent protein family have become indispensable imaging tools for cell biology. A wide variety of these proteins were discovered in nonbioluminescent anthozoa in recent years. Some of them feature exciting new properties, with the possibility to alter their intensity and/or fluorescence color by irradiation with light of specific wavelengths. Fluorescent highlighter proteins enable many interesting applications based on regional optical marking in live cells and tissues. This review provides an overview of photoactivatable marker proteins, with a focus on EosFP, a protein that can be switched from green to red fluorescence by approximately 400-nm light. A variety of applications are presented to illustrate the versatility of EosFP in live-cell imaging.

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页码：361 / 374

页数：14

相关论文

共 75 条

[1] An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein [J].

Ando, R ;

Hama, H ;

Yamamoto-Hino, M ;

Mizuno, H ;

Miyawaki, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (20) :12651-12656

[2] Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting [J].

Ando, R ;

Mizuno, H ;

Miyawaki, A .

SCIENCE, 2004, 306 (5700) :1370-1373

[3] Structure and mechanism of the reversible photoswitch of a fluorescent protein [J].

Andresen, M ;

Wahl, MC ;

Stiel, AC ;

Gräter, F ;

Schäfer, LV ;

Trowitzsch, S ;

Weber, G ;

Eggeling, C ;

Grubmüller, H ;

Hell, SW ;

Jakobs, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (37) :13070-13074

[4] Frequent fusion and fission of plant mitochondria with unequal nucleoid distribution [J].

Arimura, S ;

Yamamoto, J ;

Aida, GP ;

Nakazono, M ;

Tsutsumi, N .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (20) :7805-7808

[5] Structural basis for dual excitation and photoisomerization of the Aequorea victoria green fluorescent protein [J].

Brejc, K ;

Sixma, TK ;

Kitts, PA ;

Kain, SR ;

Tsien, RY ;

Ormo, M ;

Remington, SJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (06) :2306-2311

[6] A genetically encoded photosensitizer [J].

Bulina, ME ;

Chudakov, DM ;

Britanova, OV ;

Yanushevich, YG ;

Staroverov, DB ;

Chepurnykh, TV ;

Merzlyak, EM ;

Shkrob, MA ;

Lukyanov, S ;

Lukyanov, KA .

NATURE BIOTECHNOLOGY, 2006, 24 (01) :95-99

[7] A monomeric red fluorescent protein [J].

Campbell, RE ;

Tour, O ;

Palmer, AE ;

Steinbach, PA ;

Baird, GS ;

Zacharias, DA ;

Tsien, RY .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (12) :7877-7882

[8]

Chalfie M, 2006, METHOD BIOCHEM ANAL, V47, P1

[9] GREEN FLUORESCENT PROTEIN AS A MARKER FOR GENE-EXPRESSION [J].

CHALFIE, M ;

TU, Y ;

EUSKIRCHEN, G ;

WARD, WW ;

PRASHER, DC .

SCIENCE, 1994, 263 (5148) :802-805

[10] Ultra-fast excited state dynamics in green fluorescent protein: Multiple states and proton transfer [J].

Chattoraj, M ;

King, BA ;

Bublitz, GU ;

Boxer, SG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (16) :8362-8367

← 1 2 3 4 5 6 7 8 →