Development of a sensitive and selective LC/MS/MS method for the simultaneous determination of intracellular 1-beta-D-arabinofuranosylcytosine triphosphate (araCTP), cytidine triphosphate (CTP) and deoxycytidine triphosphate (dCTP) in a human follicular lymphoma cell line

被引：32

作者：

Crauste, Celine ^{[1
]}

Lefebvre, Isabelle ^{[1
]}

Hovaneissian, Michael ^{[1
]}

Puy, Jean Yves ^{[1
]}

Roy, Beatrice ^{[1
]}

Peyrottes, Suzanne ^{[1
]}

Cohen, Sabine ^{[2
]}

Guitton, Jerome ^{[2
]}

Dumontet, Charles ^{[3
]}

Perigaud, Christian ^{[1
]}

机构：

[1] Univ Montpellier 2, Inst Biomol Max Mousseron, UMR 5247, CNRS,UM1,UM2, F-34095 Montpellier 05, France

[2] Hop Edouard Herriot, Pharmacol Lab, Hosp Civils Lyon, F-69437 Lyon, France

[3] Hosp Civils Lyon, INSERM, U590, F-69008 Lyon, France

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2009年 / 877卷 / 14-15期

关键词：

araCTP; CTP; LC/MS/MS; WAX; Porous graphitic carbon; PERFORMANCE LIQUID-CHROMATOGRAPHY; TANDEM MASS-SPECTROMETRY; POROUS GRAPHITIC CARBON; CYTOSINE-ARABINOSIDE; PERIPHERAL-BLOOD; QUANTITATIVE-ANALYSIS; DRUG-RESISTANCE; LEUKEMIC-CELLS; METABOLITES; PHOSPHATE;

D O I：

10.1016/j.jchromb.2009.02.071

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method was developed for the quantification of araCTP, CTP and dCTP ill a human follicular lymphoma cell line. This method involves solid phase extraction (SPE) using a weak anion-exchanger (WAX) cartridge, a porous graphitic carbon high-performance liquid chromatography (HPLC) column separation, and tandem mass spectrometry (MS/MS) detection. By using a triple quadrupole mass spectrometer operating in negative ion multiple reaction monitoring (MRM) mode, the method was able to achieve a lower limit of quantification (LLOQ) of 0.1 mu g mL(-1) for araCTP and of 0.01 mu g mL(-1) for both CTP and dCTP. The method was validated and used to determine the amount of araCTP, CTP and dCTP formed after incubation of araC and an araCMP prodrug in the human follicular lymphoma cell line RL. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：1417 / 1425

页数：9

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