A recombinant RNA polymerase II-like enzyme capable of promoter-specific transcription

被引：115

作者：

Werner, F ^{[1
]}

Weinzierl, ROJ ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Dept Biol Sci, London SW7 2AZ, England

来源：

MOLECULAR CELL | 2002年 / 10卷 / 03期

基金：

英国惠康基金;

关键词：

D O I：

10.1016/S1097-2765(02)00629-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RNA polymerases (RNAPs) are core components of the cellular transcriptional machinery. Progress with functional studies of eukaryotic RNAPs has been delayed by the fact that it has not yet been possible to assemble active enzymes from individual subunits. Archaeal RNAPs are directly comparable to eukaryotic RNAPII in terms of primary sequence homology and quaternary structure. Here we report the successful in vitro assembly of a recombinant archaeal RNAP from purified subunits. The recombinant enzyme displays full activity in transcription assays and is capable, in the presence of two other basal factors, of promoter-specific transcription. The assembly of mutant enzymes yielded several unexpected insights into the structural and functional contributions of various subunits toward overall RNAP activity.

引用

页码：635 / 646

页数：12

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