Response of nitrogen fixation in relation to nodule carbohydrate metabolism in Medicago ciliaris lines subjected to salt stress

被引:31
作者
Ben Salah, Imene [1 ]
Albacete, Alfonso [2 ]
Andujar, Cristina Martinez [2 ]
Haouala, Rabiaa [3 ]
Labidi, Nehla [1 ]
Zribi, Fethia [1 ]
Martinez, Vicente [2 ]
Perez-Alfocea, Francisco [2 ]
Abdelly, Chedly [1 ]
机构
[1] CBBC, Lab Adaptat Plantes Stress Abiot, Hammam Lif 2050, Tunisia
[2] CEBAS CSIC, Dept Plant Nutr, Murcia 30100, Spain
[3] Inst Super Biotechnol Monastir, Dept Biol, Monastir 5000, Tunisia
关键词
Medicago ciliaris; Nitrogen fixation; Nodule carbon metabolism; Salt stress; Sucrose transport; BEAN PHASEOLUS-VULGARIS; SOYBEAN ROOT-NODULES; SUCROSE METABOLISM; N-2; FIXATION; SUCROLYTIC ACTIVITIES; PLANT-GROWTH; CARBON; PHOTOSYNTHATE; TOLERANCE; CHLOROPHYLL;
D O I
10.1016/j.jplph.2008.06.016
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The effect of salt stress on nitrogen fixation, in relation to sucrose transport towards nodules and other sink organs and the potential of sucrose breakdown by nodules, was investigated in two lines of Medicago ciliaris. Under salt stress conditions, the two lines showed a decrease of total biomass production, but TNC 1.8 was less affected by salt than TNC 11.9. The chlorophyll content was not changed in TNC 1.8, in contrast to TNC 11.9. Shoot, root, and nodule biomass were also affected in the two lines, but TNC 1.8 exhibited the higher potentialities of biomass production of these organs. Nitrogen fixation also decreased in the two lines, and was more sensitive to salt than growth parameters. TNC 1.8 consistently exhibited the higher values of nitrogen fixation. Unlike nodules, leaves of both lines were well. supplied in nutrients with some exceptions. Specifically, the calcium content decreased in the sensitive tine leaves, and the nodule magnesium content was not changed in either line. The tolerant line accumulated more sodium in its leaves. The two lines did not show any differences in the nodule sodium content. Sucrose allocation towards nodules was affected by salt in the two lines, but this constraint did not seem to affect the repartition of sucrose between sink organs. Salt stress induced perturbations in nodule sucrolytic activities in the two tines. It inhibited sucrose synthase, but the inhibition was more marked in TNC 11.9; alkaline/neutral activity was not altered in TNC 1.8, whereas it decreased more than half in TNC 11.9. Thus, the relative tolerance of TNC 1.8 to salt stress could be attributed to a better use of these photoassimilates by nodules and a better supply of bacteroids in malate. The hypothesis of a competition for sucrose between nodules and other sink organs under salt stress could not be verified. (C) 2008 Elsevier GmbH. All rights reserved.
引用
收藏
页码:477 / 488
页数:12
相关论文
共 54 条
[21]  
Hewitt E., 1966, SAND WATER CULTURE M
[22]  
JANEZ HB, 1997, NEW PHYTOL, V137, P257
[23]   Changes in ascorbate peroxidase, catalase, guaiacol peroxidase and superoxide dismutase activities in common bean (Phaseolus vulgaris) nodules under salt stress [J].
Jebara, S ;
Jebara, M ;
Limam, F ;
Aouani, ME .
JOURNAL OF PLANT PHYSIOLOGY, 2005, 162 (08) :929-936
[24]   SALT STRESS-INDUCED CYTOPLASMIC ACIDIFICATION AND VACUOLAR ALKALIZATION IN NITELLOPSIS-OBTUSA CELLS - INVIVO P-31-NUCLEAR MAGNETIC-RESONANCE STUDY [J].
KATSUHARA, M ;
KUCHITSU, K ;
TAKESHIGE, K ;
TAZAWA, M .
PLANT PHYSIOLOGY, 1989, 90 (03) :1102-1107
[25]  
Kjeldahl J., 1883, Z ANAL CHEM, V22, P366, DOI [10.1007/BF01338151, DOI 10.1007/BF01338151]
[26]   Sucrose metabolism: regulatory mechanisms and pivotal roles in sugar sensing and plant development [J].
Koch, K .
CURRENT OPINION IN PLANT BIOLOGY, 2004, 7 (03) :235-246
[27]   DYNAMICS OF CARBON PHOTOSYNTHETICALLY ASSIMILATED IN NODULATED SOYA BEAN-PLANTS UNDER STEADY-STATE CONDITIONS .3. TIME-COURSE STUDY ON CO2(C-13) INCORPORATION INTO SOLUBLE METABOLITES AND RESPIRATORY EVOLUTION OF C-13 FROM ROOTS AND NODULES [J].
KOUCHI, H ;
NAKAJI, K ;
YONEYAMA, T ;
ISHIZUKA, J .
ANNALS OF BOTANY, 1985, 56 (03) :333-346
[28]  
LOPEZ M, 2007, J PLANT PHYSL
[29]   Differences in responses to iron deficiency between two legumes:: lentil (Lens culinaris) and chickpea (Cicer arietinum) [J].
Mahmoudi, H ;
Ksouri, R ;
Gharsalli, M ;
Lachaâl, M .
JOURNAL OF PLANT PHYSIOLOGY, 2005, 162 (11) :1237-1245
[30]   Molecular mechanisms of potassium and sodium uptake in plants [J].
Mäser, P ;
Gierth, M ;
Schroeder, JI .
PLANT AND SOIL, 2002, 247 (01) :43-54