High-throughput protein arrays:: prospects for molecular diagnostics

被引：68

作者：

Walter, G

Büssow, K

Lueking, A

Glökler, J

机构：

[1] Cornupia Capital Ltd, Biorchard Ltd, London WC1N 3XX, England

[2] Max Planck Inst Mol Genet, DE-14195 Berlin, Germany

[3] Scienion AG, DE-12489 Berlin, Germany

来源：

TRENDS IN MOLECULAR MEDICINE | 2002年 / 8卷 / 06期

关键词：

D O I：

10.1016/S1471-4914(02)02352-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

High-throughput protein arrays allow the miniaturized and parallel analysis of large numbers of diagnostic markers in complex samples, Using automated colony picking and gridding, cDNA or antibody libraries can be expressed and screened as clone arrays. Protein microarrays are constructed from recombinantly expressed, purified, and yet functional proteins, entailing a range of optimized expression systems. Antibody microarrays are becoming a robust format for expression profiling of whole genomes. Alternative systems, such as aptamer, PROfusion(TM), nano- and microfluidic arrays are all at proof-of-concept stage. Differential protein profiles have been used as molecular diagnostics for cancer and autoimmune diseases and might ultimately be applied to screening of high-risk and general populations.

引用

页码：250 / 253

页数：4

共 31 条

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