Electrochemical studies of o-tilidine as a hydrogen donor substrate for peroxidase and its application in enzyme immunoassay

被引:14
作者
Jiao, K [1 ]
Sun, W [1 ]
机构
[1] Oingdao Inst Chem Technol, Dept Appl Chem, Shandong 266042, Peoples R China
基金
中国国家自然科学基金;
关键词
o-tilidine; horseradish peroxidase; enzyme immunoassay; cucumber mosaic virus; voltammetry;
D O I
10.1016/S0026-265X(01)00116-3
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A highly sensitive electrochemical method is described for assay of horseradish peroxidase (HRP) using o-tilidine as substrate. Under the optimal conditions, the detection limit for HRP is 2.5 mU/l and the calibration range is from 5.0 to 1000 mU/l. The relative standard deviation of 11 measurements is 6.3% for 10.0 mU/l HRP. This new electrochemical system was further combined with an indirect enzyme immunoassay using direct antigen-coating format for the detection of cucumber mosaic virus (CMV). The results show an improved sensitivity over the traditional o-phenylenediamine (OPD) spectrophotometric enzyme-linked immunosorbent assay (ELISA) method. Using this technique, a minimum detectable level of 2.0 ng/ml of purified CMV and 1:12500 dilution of CMV in infected leaf extractions can be achieved. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:123 / 130
页数:8
相关论文
共 23 条
  • [21] Zhang SS, 1999, ELECTROANAL, V11, P511, DOI 10.1002/(SICI)1521-4109(199906)11:7<511::AID-ELAN511>3.0.CO
  • [22] 2-V
  • [23] Detection of ferritin in human serum with a MAP-H2O2-HRP voltammetric enzyme-linked immunoassay system
    Zhang, SS
    Jiao, K
    Chen, HY
    Wang, MX
    [J]. TALANTA, 1999, 50 (01) : 95 - 101