Salt and osmotic stress cause rapid increases in Arabidopsis thaliana cGMP levels

被引：120

作者：

Donaldson, L

Ludidi, N

Knight, MR

Gehring, C

Denby, K ^{[1
]}

机构：

[1] Univ Cape Town, Dept Mol & Cell Biol, ZA-7701 Rondebosch, South Africa

[2] Univ Western Cape, Dept Biotechnol, ZA-7535 Bellville, South Africa

[3] Univ Oxford, Dept Plant Sci, Oxford OX1 3RB, England

来源：

FEBS LETTERS | 2004年 / 569卷 / 1-3期

关键词：

NaCl stress; osmotic stress; cGMP; cytosolic calcium; second messenger; Arabidopsis thaliana;

D O I：

10.1016/j.febslet.2004.06.016

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A guanylyl cyclase has been recently identified in Arabidopsis but, despite the use of pharmacological inhibitors to infer roles of the second messenger 3',5'-cyclic guanosine monophosphate (cGMP), very few measurements of actual cGMP levels in plants are available. Here, we demonstrate that cGMP levels in Arabidopsis seedlings increase rapidly (less than or equal to 5 s) and to different degrees after salt and osmotic stress, and that the increases are prevented by treatment with LY, an inhibitor of soluble guanylyl cyclases. In addition, we provide evidence to suggest that salt stress activates two cGMP signalling pathways an osmotic, calcium-independent pathway and an ionic, calcium-dependent pathway. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：317 / 320

页数：4

共 23 条

[1] HLM1, an essential signaling component in the hypersensitive response, is a member of the cyclic nucleotide-gated channel ion channel family [J].