The SH3 domain of the tight junction protein ZO-1 binds to a serine protein kinase that phosphorylates a region C-terminal to this domain

被引：84

作者：

Balda, MS ^{[1
]}

Anderson, JM ^{[1
]}

Matter, K ^{[1
]}

机构：

[1] YALE UNIV, SCH MED, DEPT INTERNAL MED & CELL BIOL, NEW HAVEN, CT USA

来源：

FEBS LETTERS | 1996年 / 399卷 / 03期

关键词：

tight junction; ZO-1; SH3; domain; serine protein kinase;

D O I：

10.1016/S0014-5793(96)01352-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

ZO-1 is a tight junction phosphoprotein partially homologous to a tumor suppressor in Drosophila. The homologous region contains an SH3 domain with an unidentified function. Using fusion proteins containing the SH3 domain and various N- and C-terminal sequences, we tested for association of a kinase with this protein domain in extracts of MDCK cells. We show that the SH3 domain of ZO-1 binds a serine protein kinase that phosphorylates a region immediately C-terminal to the SH3 domain. This kinase associates specifically with the SH3 domain of ZO-1 and appears to be also associated with junctional complexes extracted from MDCK cells.

引用

页码：326 / 332

页数：7