Preparation of antibody-conjugated gold nanoparticles

被引：56

作者：

Di Pasqua, Anthony J. ^{[1
]}

Mishler, Richard E., II ^{[1
]}

Ship, Yan-Li ^{[1
]}

Dabrowiak, James C. ^{[1
]}

Asefa, Tewodros ^{[1
]}

机构：

[1] Syracuse Univ, Dept Chem, CST, Syracuse, NY 13244 USA

来源：

MATERIALS LETTERS | 2009年 / 63卷 / 21期

关键词：

Gold nanoparticles; E; coli; Pathogen; Antibody; ESCHERICHIA-COLI O157-H7; ENZYME;

D O I：

10.1016/j.matlet.2009.05.070

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

We here describe a method for the simple synthesis of gold nanoparticles (similar to 10 nm in diameter) conjugated with antibody to E. coli O157:H7. Gold nanoparticles with pendant carboxylic acid and alcohol functional groups were synthesized and characterized using transmission electron microscopy (TEM) and infrared spectroscopy. These nanoparticles were then reacted with anti-E. coli O157:H7, using EDC coupling chemistry, and the product was characterized with X-ray photoelectron spectroscopy. Furthermore, binding of the antibody-gold conjugates to E. coli O157:H7 was demonstrated using transmission electron microscopy. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：1876 / 1879

页数：4

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