Preparation of antibody-conjugated gold nanoparticles

被引:56
作者
Di Pasqua, Anthony J. [1 ]
Mishler, Richard E., II [1 ]
Ship, Yan-Li [1 ]
Dabrowiak, James C. [1 ]
Asefa, Tewodros [1 ]
机构
[1] Syracuse Univ, Dept Chem, CST, Syracuse, NY 13244 USA
关键词
Gold nanoparticles; E; coli; Pathogen; Antibody; ESCHERICHIA-COLI O157-H7; ENZYME;
D O I
10.1016/j.matlet.2009.05.070
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
We here describe a method for the simple synthesis of gold nanoparticles (similar to 10 nm in diameter) conjugated with antibody to E. coli O157:H7. Gold nanoparticles with pendant carboxylic acid and alcohol functional groups were synthesized and characterized using transmission electron microscopy (TEM) and infrared spectroscopy. These nanoparticles were then reacted with anti-E. coli O157:H7, using EDC coupling chemistry, and the product was characterized with X-ray photoelectron spectroscopy. Furthermore, binding of the antibody-gold conjugates to E. coli O157:H7 was demonstrated using transmission electron microscopy. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码:1876 / 1879
页数:4
相关论文
共 21 条
[1]   Synthesis of gold nanoparticles via electroless deposition in SBA-15 [J].
Asefa, T ;
Lennox, RB .
CHEMISTRY OF MATERIALS, 2005, 17 (10) :2481-2483
[2]  
AZIOUNE A, 2003, EUR CELLS MATER, V6, P85
[3]   Direct oriented immobilization of F(ab′) antibody fragments on gold [J].
Brogan, KL ;
Wolfe, KN ;
Jones, PA ;
Schoenfisch, MH .
ANALYTICA CHIMICA ACTA, 2003, 496 (1-2) :73-80
[4]   Waterborne Escherichia coli O157 [J].
Chalmers, RM ;
Aird, H ;
Bolton, FJ .
JOURNAL OF APPLIED MICROBIOLOGY, 2000, 88 :124S-132S
[5]   Synthesis and characterization of carboxylate-modified gold nanoparticle powders dispersible in water [J].
Chen, SH ;
Kimura, K .
LANGMUIR, 1999, 15 (04) :1075-1082
[6]  
Chen Si Chen Si, 2007, Chinese Journal of Agricultural Biotechnology, V4, P15, DOI 10.1017/S1479236207001349
[7]   Surface modification of gold nanorods through a place exchange reaction inside an ionic exchange resin [J].
Dai, Qiu ;
Coutts, Janelle ;
Zou, Jianhua ;
Huo, Qun .
CHEMICAL COMMUNICATIONS, 2008, (25) :2858-2860
[8]   Strategies for the detection of Escherichia coli O157:H7 in foods [J].
Deisingh, AK ;
Thompson, M .
JOURNAL OF APPLIED MICROBIOLOGY, 2004, 96 (03) :419-429
[9]   Comparison of enzyme-linked immunomagnetic chemiluminescence with US Food and Drug Administration's Bacteriological Analytical Manual method for the detection of Escherichia coli O157:H7 [J].
Gehring, Andrew G. ;
Albin, David M. ;
Irwin, Peter L. ;
Reed, Sue A. ;
Tu, Shu-I .
JOURNAL OF MICROBIOLOGICAL METHODS, 2006, 67 (03) :527-533
[10]   Functionalized gold nanoparticles mimic catalytic activity of a polysiloxane-synthesizing enzyme [J].
Kisailus, D ;
Najarian, M ;
Weaver, JC ;
Morse, DE .
ADVANCED MATERIALS, 2005, 17 (10) :1234-+