Ca2+ signaling evoked by activation of Na+ channels and Na+/Ca2+ exchangers is required for GABA-induced NG2 cell migration

被引:109
作者
Tong, Xiao-ping
Li, Xiang-yao
Zhou, Bing
Shen, Wanhua
Zhang, Zhi-jun
Xu, Tian-le
Duan, Shumin [1 ]
机构
[1] Chinese Acad Sci, Inst Neurosci, Shanghai 200031, Peoples R China
基金
中国国家自然科学基金;
关键词
OLIGODENDROCYTE PRECURSOR CELLS; GIANT DEPOLARIZING POTENTIALS; POSTNATAL SUBVENTRICULAR ZONE; CENTRAL-NERVOUS-SYSTEM; CNS WHITE-MATTER; RAT OPTIC-NERVE; NEURONAL MIGRATION; MULTIPLE-SCLEROSIS; ADHESION MOLECULE; PROGENITOR CELLS;
D O I
10.1083/jcb.200811071
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
NG2 cells originate from various brain regions and migrate to their destinations during early development. These cells express voltage-gated Na+ channels but fail to produce typical action potentials. The physiological role of Na+ channels in these cells is unclear. We found that GABA induces membrane depolarization and Ca2+ elevation in NG2 cells, a process requiring activation of GABA(A) receptors, Na+ channels, and Na+/Ca2+ exchangers (NCXs), but not Ca2+ channels. We have identified a persistent Na+ current in these cells that may underlie the GABA-induced pathway of prolonged Na+ elevation, which in turn triggers Ca2+ influx via NCXs. This unique Ca2+ signaling pathway is further shown to be involved in the migration of NG2 cells. Thus, GABAergic signaling mediated by sequential activation of GABA(A) receptors, noninactivating Na+ channels, and NCXs may play an important role in the development and function of NG2 glial cells in the brain.
引用
收藏
页码:113 / 128
页数:16
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