Distinct contributions of CD4+ and CD8+ naive and memory T-cell subsets to overall T-cell-receptor repertoire complexity following transplantation of T-cell-depleted CD34-selected hematopoietic progenitor cells from unrelated donors

被引:22
作者
Eyrich, M
Croner, T
Leiler, C
Lang, P
Bader, P
Klingebiel, T
Niethammer, D
Schlegel, PG
机构
[1] Goethe Univ Frankfurt, Dept Pediat Hematol Oncol, Childrens Hosp, D-6000 Frankfurt, Germany
[2] Univ Tubingen, Pediat Stem Cell Transplant Program, Program Project Grant IZKF, D-72076 Tubingen, Germany
关键词
D O I
10.1182/blood-2001-11-0005
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Normalization of restricted T-cell-receptor (TCR) repertoire is critical following T-cell-depleted (TCD) stem cell transplantation. We present a prospective study analyzing respective contributions of naive and memory T-cell subsets within the CD4(+) and CD8(+) compartments to the evolution of overall TCR-repertoire complexity following transplantation of CD34-selected peripheral blood progenitor cells from unrelated donors. During the first year after transplantation, sorted CD4/45RA, CD4/45R0, CD8/45RA, and CD8/45R0 subsets were analyzed at 3-month intervals for TCR-repertoire complexity by CDR3 size spectratyping. Skew in TCR- repertoire was observed only in early memory-type T cells. CD4(+) and CD8(+) subsets differed in clonal distribution of CDR3 sizes, with rapid Gaussian normalization of bands in CD4/45R0(+) T cells. Naive T cells displayed normal repertoire complexity and contributed significantly to skew correction. Our data provide direct evidence for an important role of de novo maturation of naive T cells in normalization of an initially restricted TCR-repertoire following transplantation of CD34-selected, TCD-depleted peripheral blood progenitors from unrelated donors. (C) 2002 by The American Society of Hematology.
引用
收藏
页码:1915 / 1918
页数:4
相关论文
共 22 条
[21]   Factors affecting thymic function after allogeneic hematopoietic stem cell transplantation [J].
Weinberg, K ;
Blazar, BR ;
Wagner, JE ;
Agura, E ;
Hill, BJ ;
Smogorzewska, M ;
Koup, RA ;
Betts, MR ;
Collins, RH ;
Douek, DC .
BLOOD, 2001, 97 (05) :1458-1466
[22]  
Wu CJ, 2000, BLOOD, V95, P352