Enhanced triterpene and phytosterol biosynthesis in Panax ginseng overexpressing squalene synthase gene

被引:309
作者
Lee, MH
Jeong, JH
Seo, JW
Shin, CG
Kim, YS
In, JG
Yang, DC
Yi, JS
Choi, YE [1 ]
机构
[1] Kangwon Natl Univ, Div Forest Resources, Coll Forest Sci, Chunchon, Kangwon Do, South Korea
[2] Chung Ang Univ, Korea Ginseng Inst, Ansung 456756, Kyunggi Do, South Korea
[3] Kumho Petrochem Co, Kumho Life & Environm Sci Lab, Kwangju 500712, South Korea
[4] Kyung Hee Univ, Dept Oriental Med Mat & Proc, Suwon 449701, Kyunggi Do, South Korea
基金
新加坡国家研究基金会;
关键词
ginsenoside; methyl jasmonate; Panax ginseng; phytosterol squalene synthase (EC2.5.1.21); triterpene saponin;
D O I
10.1093/pcp/pch126
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Roots of Panax ginseng, one of the most famous medicinal plants, contain various phytosterols and bioactive triterpene saponins (ginsenosides). In P ginseng, phytosterols and triterpenes share the common biosynthetic intermediate, squalene. Here, we investigate the regulatory role of Panax ginseng squalene synthase (PgSS1) on the biosynthesis of phytosterols and triterpene saponins. PgSS1 transcripts are expressed ubiquitously in the various plant tissues, but higher in shoot apex and root. The transcript levels of PgSS1 increased markedly in the adventitious roots during 12- to 96-h period after metyl jasmonate (MeJA) treatment; MeJA treatment induced the activation of the transcripts of squalene epoxidase (SE), P-amyrin synthase (bAS), but not cycloartenol synthase (CAS). Unlike MeJA treatment, overexpression of PgSS1 in adventitious roots of transgenic P ginseng was followed by the up-regulation of all the downstream genes tested, such as SE, bAS, and CAS. The enhanced activity of PgSS1 enzyme resulted in remarkable increase of phytosterols as well as ginsenoside contents. These results demonstrate that PgSS1 is a key regulatory enzyme not only for phytosterol but also for triterpene biosynthesis and overexpressing of PgSS1 confers the hyperproduction of triterpene saponins to P ginseng.
引用
收藏
页码:976 / 984
页数:9
相关论文
共 40 条
[11]   Natural products and plant disease resistance [J].
Dixon, RA .
NATURE, 2001, 411 (6839) :843-847
[12]   Legume natural products: Understanding and manipulating complex pathways for human and animal health [J].
Dixon, RA ;
Sumner, LW .
PLANT PHYSIOLOGY, 2003, 131 (03) :878-885
[13]   Biosynthesis of sterols and triterpenes in cell suspension cultures of Uncaria tomentosa [J].
Flores-Sánchez, IJ ;
Ortega-López, J ;
Montes-Horcasitas, MD ;
Ramos-Valdivia, AC .
PLANT AND CELL PHYSIOLOGY, 2002, 43 (12) :1502-1509
[14]   JASMONIC ACID IS A SIGNAL TRANSDUCER IN ELICITOR-INDUCED PLANT-CELL CULTURES [J].
GUNDLACH, H ;
MULLER, MJ ;
KUTCHAN, TM ;
ZENK, MH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (06) :2389-2393
[15]  
HARTMANN MA, 1987, METHOD ENZYMOL, V148, P632
[16]   Up-regulation of soyasaponin biosynthesis by methyl jasmonate in cultured cells of Glycyrrhiza glabra [J].
Hayashi, H ;
Huang, PY ;
Inoue, K .
PLANT AND CELL PHYSIOLOGY, 2003, 44 (04) :404-411
[17]  
Hostettmann K., 1995, SAPONINS
[18]   Dietary phytosterols as cholesterol-lowering agents in humans [J].
Jones, PJH ;
MacDougall, DE ;
Ntanios, F ;
Vanstone, CA .
CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 1997, 75 (03) :217-227
[19]   Discovery of genes for ginsenoside biosynthesis by analysis of ginseng expressed sequence tags [J].
Jung, JD ;
Park, HW ;
Hahn, Y ;
Hur, CG ;
In, DS ;
Chung, HJ ;
Liu, JR ;
Choi, DW .
PLANT CELL REPORTS, 2003, 22 (03) :224-230
[20]   CHANGES IN TRITERPENOID CONTENT DURING THE GROWTH-CYCLE OF CULTURED PLANT-CELLS [J].
KAMISAKO, W ;
MORIMOTO, K ;
MAKINO, I ;
ISOI, K .
PLANT AND CELL PHYSIOLOGY, 1984, 25 (08) :1571-1574